Phospho-Chk1 (Ser317) Polyclonal antibody
Phospho-Chk1 (Ser317) Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | UV treated HEK-293T cells |
Planning an IHC experiment? We recommend our IHCeasy CHK1 Ready-To-Use IHC Kit. CHK1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
28807-1-AP targets Phospho-Chk1 (Ser317) in WB, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | CHK1 checkpoint homolog (S. pombe) |
| Synonyms | CHEK1, CHK1, Phospho-CHK1 (Ser317) |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC004202 |
| Gene Symbol | CHEK1 |
| Gene ID (NCBI) | 1111 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14757 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The checkpoint kinase 1 (Chk1) is a conserved kinase that imposes cell cycle delays in response to impediments to DNA replication. Chk1 kinase is downstream of the ATR kinase. ATR phosphorylates Chk1 kinase and other proteins to ensure replication is being blocked as to avoid replication fork collapse and DNA damage. Activation of Chk1 involves phosphorylation at Ser317 and Ser345 by ATM/ATR, followed by autophosphorylation of Ser296. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases. (PMID:22941630, PMID: 32571801, PMID:19276361)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-Chk1 (Ser317) antibody 28807-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Antiviral Res Proteomic and phosphoproteomic analysis of responses to enterovirus A71 infection reveals novel targets for antiviral and viral replication |