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验证数据展示

  • WB analysis of mouse heart using 30937-1-AP

    WB analysis of mouse heart using 30937-1-AP

    Phosphatase inhibitor treated mouse heart tissue, λ phosphatase treated mouse heart tissue, and non-treated mouse heart tissue were subjected to SDS PAGE followed by western blot with 30937-1-AP (Phospho-TNNI3 (Ser23/24) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

  • FC experiment of mouse heart using 30937-1-AP

    FC experiment of mouse heart using 30937-1-AP

    1x10^6 λ phosphatase treated mouse heart tissue were intracellularly stained with 0.13 ug Phospho-Cardiac Troponin I (Ser23/24) Polyclonal antibody (30937-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), and 0.13 ug Isotype Control. Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Phospho-Cardiac Troponin I (Ser23/24) Polyclonal antibody

Phospho-Cardiac Troponin I (Ser23/24) Polyclonal Antibody for WB, FC (Intra), ELISA
Cat No. 30937-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

Cardiac Troponin I (Ser23/24), p Cardiac Troponin I, p Cardiac Troponin I (Ser23/24), p Cardiac Troponin I Ser23/24, P-Cardiac Troponin I

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue, λ phosphatase treated mouse heart tissue
Positive FC (Intra) detected inmouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy TNNI3 Ready-To-Use IHC Kit. TNNI3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30937-1-AP targets Phospho-Cardiac Troponin I (Ser23/24) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 troponin I type 3 (cardiac)
别名 Cardiac Troponin I (Ser23/24), p Cardiac Troponin I, p Cardiac Troponin I (Ser23/24), p Cardiac Troponin I Ser23/24, P-Cardiac Troponin I
计算分子量 210 aa, 24 kDa
观测分子量26 kDa
GenBank蛋白编号BC096165
基因名称 Cardiac Troponin I
Gene ID (NCBI) 7137
RRIDAB_3669787
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP19429
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cardiac Troponin I (cTnI) is produced by cardiac muscle. To date, most investigative effort in phosphorylation mediated regulation of cTnI function has focused on the actions of PKA and PKC. Evidence from studies in a variety of systems, ranging from reconstituted myofilament proteins to cultured myocytes or transgenic animals with heterologous expression of modified cTnI proteins, indicates that PKA-mediated functional effects (eg, decreased myofilament Ca2 sensitivity) are mediated principally through the phosphorylation of Ser22/23 (equivalent to Ser23/24, including the initiating Met, in the rodent sequence) within the unique N-terminal extension of cTnI. (PMID: 15514163)

实验方案

Product Specific Protocols
FC protocol for Phospho-Cardiac Troponin I (Ser23/24) antibody 30937-1-APDownload protocol
WB protocol for Phospho-Cardiac Troponin I (Ser23/24) antibody 30937-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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