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验证数据展示

  • WB analysis using 80084-1-RR

    WB analysis using 80084-1-RR

    Lambda protein phosphatase treated HEK-293 and NIH/3T3 and Non-treated HEK-293 and NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80084-1-RR (Phospho-Beta Catenin (Ser675) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was re-blotted with Beta Catenin antibody 66379-1-Ig as control.

  • IF Staining of MCF-7 using 80084-1-RR

    IF Staining of MCF-7 using 80084-1-RR

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated MCF-7 cells using Phospho-Beta Catenin (Ser675) antibody (80084-1-RR, Clone: 6D6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • IF Staining of MCF-7 using 80084-1-RR

    IF Staining of MCF-7 using 80084-1-RR

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated MCF-7 cells using Phospho-Beta Catenin (Ser675) antibody (80084-1-RR, Clone: 6D6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

Phospho-Beta Catenin (Ser675) Recombinant monoclonal antibody

Phospho-Beta Catenin (Ser675) Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 80084-1-RR

产品说明书

CloneNo. 6D6
引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat and More (1)

应用

WB, IF/ICC, ELISA

别名

b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, λ phosphatase treated HEK-293, λ phosphatase treated NIH/3T3 cells
Positive IF/ICC detected inλ phosphatase treated MCF-7 cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy Beta Catenin Ready-To-Use IHC Kit. Beta Catenin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

80084-1-RR targets Phospho-Beta Catenin (Ser675) in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, mouse, rat
文献引用反应性human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 catenin (cadherin-associated protein), beta 1, 88kDa
别名 b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286
计算分子量 781 aa, 86 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC058926
基因名称 Beta Catenin
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
RRIDAB_2918863
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35222
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222). PKA was shown to phosphorylate β-catenin at Ser675. Phosphorylation at Ser675 induces β-catenin accumulation in the nucleus and increases its transcriptional activity.

实验方案

Product Specific Protocols
WB protocol for Phospho-Beta Catenin (Ser675) antibody 80084-1-RRDownload protocol
IF protocol for Phospho-Beta Catenin (Ser675) antibody 80084-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Ginsenoside Rc Promotes Bone Formation in Ovariectomy-Induced Osteoporosis In Vivo and Osteogenic Differentiation In Vitro.

Authors - Nan Yang
View Article
mouseWB

Mol Neurobiol

GPR30 Inhibits Neuronal Apoptosis After Subarachnoid Hemorrhage by Activating the Wnt/β-Catenin Pathway in a m6A-dependent Manner

Authors - Jun Peng
View Article
humanWB

Nat Commun

Cytoplasmic TRIM24 promotes colorectal cancer cell proliferation by activating Wnt/β-catenin signaling

Authors - Ya Wang
View Article