验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, λ phosphatase treated mouse brain tissue |
Planning an IHC experiment? We recommend our IHCeasy DPYSL2 Ready-To-Use IHC Kit. DPYSL2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87647-3-RR targets Phospho-CRMP2 (Thr514) in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | dihydropyrimidinase-like 2 |
| 别名 | CRMP2 (phospho T514), CRMP2 (Thr514), CRMP-2 (Thr514), p-CRMP2, p-CRMP2 (Thr514) |
| 计算分子量 | 62 kDa |
| 观测分子量 | 60-80 kDa |
| GenBank蛋白编号 | NM_001386 |
| 基因名称 | CRMP2 |
| Gene ID (NCBI) | 1808 |
| RRID | AB_3745674 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q16555 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Dihydropyrimidinase-like 2 (Dpysl2), a protein involved in neuronal differentiation and axonal guidance is found altered in various nervous system diseases. It is also named as CRMP2, ULIP2 and belongs to hydantoinase/dihydropyrimidinase subfamily. Phospho-CRMP2 (Thr514) refers to the phosphorylated form of Collapsin Response Mediator Protein 2 (CRMP2) at threonine residue 514. CRMP2 is a cytosolic phosphoprotein highly expressed in the nervous system that regulates microtubule dynamics, axonal growth, and neuronal polarity. (PMID: 27609071)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-CRMP2 (Thr514) antibody 87647-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
