验证数据展示
经过测试的应用
| Positive WB detected in | Ramos cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87153-1-RR targets Phospho-CD19 (Tyr531) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CD19 molecule |
| 别名 | CD19 (Tyr531), p-CD19, p-CD19 (Tyr531), p-CD19(Tyr531), Phospho CD19 |
| 计算分子量 | 556 aa, 61 kDa |
| 观测分子量 | 95 kDa |
| GenBank蛋白编号 | NM_001770 |
| 基因名称 | CD19 |
| Gene ID (NCBI) | 930 |
| ENSEMBL Gene ID | ENSG00000177455 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P15391 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. Phospho-CD19 (Tyr531) is a phosphorylated form of CD19, which is a protein involved in B cell receptor (BCR) signaling and plays a crucial role in B cell development and function. Phosphorylation of CD19 at Tyr531 is known to recruit PI3K activity leading to Akt activation and attendant S473 phosphorylation. This activation of BCR induces multiple oncogenic signals and treatment with ibrutinib prevents preferentially the activation of BTK in both patients with ABC-like and GCB-like molecular features. (PMID: 20038598)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-CD19 (Tyr531) antibody 87153-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
