验证数据展示
经过测试的应用
| Positive WB detected in | Mo7e cells |
The phosphorylation of c-Kit at the Tyr721 site is also widely known as Tyr719.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30814-1-AP targets Phospho-CD117/c-Kit (Tyr721) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog |
| 别名 | CD117, KIT, C Kit, c-Kit, EC:2.7.10.1 |
| 计算分子量 | 976 aa, 110 kDa |
| 观测分子量 | 145 kDa |
| GenBank蛋白编号 | BC071593 |
| 基因名称 | CD117 |
| Gene ID (NCBI) | 3815 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P10721 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Kit is composed of five N-glycosylated immunoglobulin domains in the N-terminal extracellular portion that bind SCF, a transmembrane domain, and the carboxy-terminal intracellular tyrosine kinase domain. The binding of SCF autophosphorylates Kit on specific tyrosine residues (Tyr), such as Tyr568/570, Tyr703, Tyr721, and Tyr936. Kit then binds to other cytoplasmic enzymes, such as PI3K and Src kinases, and this complex activates downstream molecules. This activates the Akt-Bad pathway and the Ras-Mek-Erk cascade, which regulate gene expression and cytoskeletal structures, resulting in cell proliferation and survival. (PMID: 28403213)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-CD117/c-Kit (Tyr721) antibody 30814-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
