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验证数据展示

  • WB analysis using 87869-3-RR

    WB analysis using 87869-3-RR

    Non-treated HeLa cells and paclitaxel treated HeLa cells were subjected to SDS PAGE followed by western blot with 87869-3-RR (Phospho-CBX3 (Ser93) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • WB analysis using 87869-3-RR

    WB analysis using 87869-3-RR

    Non-treated NIH/3T3 cells and Forskolin treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 87869-3-RR (Phospho-CBX3 (Ser93) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • FC experiment of HeLa using 87869-3-RR

    FC experiment of HeLa using 87869-3-RR

    1x10^6 HeLa cells untreated (dashed lines) or treated with paclitaxel which intracellularly stained with 0.13 ug Phospho-CBX3 (Ser93) Recombinant monoclonal antibody (87869-3-RR, Clone:260091A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Phospho-CBX3 (Ser93) Recombinant monoclonal antibody

Phospho-CBX3 (Ser93) Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 87869-3-RR

产品说明书

CloneNo. 260091A2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

CBX3 (Ser93), HP1 gamma (Ser83), p-CBX3, p-CBX3 (Ser93), Phospho-CBX3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inForskolin treated NIH/3T3 cells, paclitaxel treated HeLa cells
Positive FC (Intra) detected inpaclitaxel treated HeLa cells

This antibody, also known as HP1 gamma Ser83.

Planning an IHC experiment? We recommend our IHCeasy CBX3 Ready-To-Use IHC Kit. CBX3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87869-3-RR targets Phospho-CBX3 (Ser93) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromobox homolog 3 (HP1 gamma homolog, Drosophila)
别名 CBX3 (Ser93), HP1 gamma (Ser83), p-CBX3, p-CBX3 (Ser93), Phospho-CBX3
计算分子量 21 kDa
观测分子量22 kDa
GenBank蛋白编号NM_016587
基因名称 CBX3
Gene ID (NCBI) 11335
RRIDAB_3745797
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13185
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CBX3, also known as HP1γ (Heterochromatin Protein 1 gamma), is a protein-coding gene located on human chromosome 7p15.2. Phospho-CBX3 (Ser93) refers to the phosphorylated form of Chromobox Homolog 3 (CBX3), also known as HP1γ (Heterochromatin Protein 1 gamma), at serine residue 93. This phosphorylation site is located in the N-terminal tail region of the protein and plays a regulatory role in chromatin binding and transcriptional control. (PMID: 39907700)

实验方案

Product Specific Protocols
FC protocol for Phospho-CBX3 (Ser93) antibody 87869-3-RRDownload protocol
WB protocol for Phospho-CBX3 (Ser93) antibody 87869-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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