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验证数据展示

  • WB analysis using 87337-2-RR

    WB analysis using 87337-2-RR

    Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 87337-2-RR (Phospho-CAD (Ser1859) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control.

  • WB analysis using 87337-2-RR

    WB analysis using 87337-2-RR

    Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 87337-2-RR (Phospho-CAD (Ser1859) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control.

  • WB analysis using 87337-2-RR

    WB analysis using 87337-2-RR

    Non-treated MCF-7 cells and Rapamycin treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 87337-2-RR (Phospho-CAD (Ser1859) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control.

  • IF Staining of MCF-7 using 87337-2-RR

    IF Staining of MCF-7 using 87337-2-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed Rapamycin treated MCF-7 cells using Phospho-CAD (Ser1859) antibody (87337-2-RR, Clone: 252640C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Phospho-CAD (Ser1859) Recombinant monoclonal antibody

Phospho-CAD (Ser1859) Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87337-2-RR

产品说明书

CloneNo. 252640C5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

CAD (Ser1859), p-CAD, p-CAD (Ser1859), Phospho-CAD, CAD

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, Rapamycin treated MCF-7 cells
Positive IF/ICC detected inRapamycin treated MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87337-2-RR targets Phospho-CAD (Ser1859) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
别名 CAD (Ser1859), p-CAD, p-CAD (Ser1859), Phospho-CAD, CAD
计算分子量 2225 aa, 243 kDa
观测分子量250 kDa
GenBank蛋白编号NM_004341
基因名称 CAD
Gene ID (NCBI) 790
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP27708
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamoylase, and dihydroorotase) is a multifunctional enzyme required for the de novo synthesis of pyrimidine nucleotides. Phospho-CAD (Ser1859) refers to the phosphorylated form of the multi-enzyme CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase) on serine 1859. This modification is catalyzed by ribosomal protein S6 kinase 1 (S6K1) downstream of mTORC1 signaling and serves as a metabolic switch that converts CAD from its basal state into a highly active oligomer, thereby accelerating the first three rate-limiting steps of de novo pyrimidine synthesis. (PMID:25422319, PMID: 32974014)

实验方案

Product Specific Protocols
IF protocol for Phospho-CAD (Ser1859) antibody 87337-2-RRDownload protocol
WB protocol for Phospho-CAD (Ser1859) antibody 87337-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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