验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, λ phosphatase treated HEK-293 cells, λ phosphatase treated HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87337-1-RR targets Phospho-CAD (Ser1859) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase |
| 别名 | CAD (Ser1859), p-CAD (S1859), p-CAD (Ser1859), p-CAD Ser1859, Phospho CAD (Ser1859) |
| 计算分子量 | 2225 aa, 243 kDa |
| 观测分子量 | 250 kDa |
| GenBank蛋白编号 | NM_004341 |
| 基因名称 | CAD |
| Gene ID (NCBI) | 790 |
| RRID | AB_3745476 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P27708 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamoylase, and dihydroorotase) is a multifunctional enzyme required for the de novo synthesis of pyrimidine nucleotides. Phospho-CAD (Ser1859) is a phosphorylated form of the CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase) enzyme at Serine 1859. This site is specifically phosphorylated by mTOR complex 1 (mTORC1), acting through the downstream kinase S6K1, and plays a role in regulating pyrimidine biosynthesis. The phosphorylation of CAD at Ser1859 is a part of the PI3K-AKT-mTORC1-S6K1 pathway that integrates growth signals to regulate cell growth, proliferation, and homeostasis by modulating several anabolic and catabolic processes.In addition, insulin also stimulates the Ser1859 phosphorylation of CAD in a manner sensitive to rapamycin as well as phosphatase and tensin homolog (PTEN) deletion in the PI3K-AKT pathway. (PMID:25422319, PMID: 34638594)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-CAD (Ser1859) antibody 87337-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
