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Phospho-Beta Catenin (Ser33) Recombinant antibody, PBS Only

Phospho-Beta Catenin (Ser33) Recombinant Antibody for WB,Indirect ELISA,FC

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB,Indirect ELISA,FC

Conjugate

Unconjugated

CloneNo.

3K1

Cat No : 80067-1-PBS

Print datasheet

Synonyms

b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286

验证数据展示

  • WB analysis using 80067-1-RR (same clone as 80067-1-PBS)

    WB analysis using 80067-1-RR (same clone as 80067-1-PBS)

    Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80067-1-RR (Phospho-Beta Catenin (Ser33)antibody) at dilution of 1:10000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with beta tubulin (66240-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 80067-1-PBS in a different storage buffer formulation.

  • FC experiment of PC-3 using 80067-1-RR (same clone as 80067-1-PBS)

    FC experiment of PC-3 using 80067-1-RR (same clone as 80067-1-PBS)

    1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.25 ug Anti-Human Phospho-Beta Catenin (Ser33) (80067-1-RR, Clone:3K1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80067-1-PBS in a different storage buffer formulation.

产品信息

80067-1-PBS targets Phospho-Beta Catenin (Ser33) in WB, FC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,Indirect ELISA,FC
Tested Reactivity Human, Mouse, Rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Synonyms b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286
Calculated Molecular Weight 781 aa, 86 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC058926
Gene Symbol CTNNB1
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35222
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222). CK1 phosphorylates β-Catenin at Ser45. This phosphorylation event primes β-Catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-Catenin protein levels and have been found in many tumor cell lines .