验证数据展示
经过测试的应用
| Positive WB detected in | pervanadate treated A172 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82816-4-RR targets Phospho-AXL (Tyr779) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | AXL receptor tyrosine kinase |
| 别名 | AXL, EC:2.7.10.1, Tyrosine-protein kinase receptor UFO, UFO |
| 计算分子量 | 894 aa, 98 kDa |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | BC032229 |
| 基因名称 | AXL |
| Gene ID (NCBI) | 558 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P30530 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Phospho-AXL (Tyr779) refers to the phosphorylated form of the receptor tyrosine kinase AXL at tyrosine 779 within its intracellular kinase domain. Upon ligand binding (e.g., Gas6 or HGF), AXL dimerizes and undergoes trans-autophosphorylation, generating phospho-Tyr779 that serves as a docking site for the p85 regulatory subunit of PI3K, thereby activating the PI3K/AKT/mTOR pathway and promoting cell survival, migration, and invasion. (PMID: 28251492)
