Phospho-AMPK Beta 1 (Ser182) Recombinant antibody

Phospho-AMPK Beta 1 (Ser182) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83924-1-RR

产品说明书

CloneNo. 240628B1

宿主/亚型

Rabbit / IgG

种属反应性

human and More (3)

应用

WB, ELISA

AMPKβ1 (Ser182), AMPKβ1Ser182, p AMPK, p AMPK Beta 1, p AMPK Beta 1 (Ser182)

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHEK-293 cells, λ phosphatase treated HEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 10 publications below

产品信息

83924-1-RR targets Phospho-AMPK Beta 1 (Ser182) in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse, rat, pig
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein kinase, AMP-activated, beta 1 non-catalytic subunit
别名 AMPKβ1 (Ser182), AMPKβ1Ser182, p AMPK, p AMPK Beta 1, p AMPK Beta 1 (Ser182)
计算分子量 38 kDa
观测分子量 38 kDa
GenBank蛋白编号BC001007
基因名称 PRKAB1
Gene ID (NCBI) 5564
RRIDAB_3671504
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9Y478
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AMPK Beta 1 (5'-AMP-activated protein kinase subunit beta-1) is also named as PRKAB1 and AMPK. AMPK, a serine/threonine kinase that exists as a heterotrimer comprised of a catalytic α-subunit and regulatory β- and γ-subunits, has been recognized as a sensor of cellular energy homeostasis (PMID: 21937710). AMPK regulates key metabolic enzymes, cell growth, apoptosis, gene transcription, and protein synthesis (PMID: 12829246). AMPK is an energy sensor and plays an essential role in the control of cellular bioenergetics by responding to various stresses including those that induce changes in the cellular AMP:ATP ratio or modulation in intracellular calcium (PMID: 27812976, PMID: 26616193). Recent studies have shown that AMPK mediates the inhibition of cell proliferation and growth of tumor cells (PMID: 16613876). AMPK also inhibits the expression of Glut1 and glycolysis in Tregs by inhibiting mTORC1 signaling (PMID: 25477880). This antibody recognizes phosphorylated AMPK Beta 1.

实验方案

Product Specific Protocols
WB protocol for Phospho-AMPK Beta 1 (Ser182) antibody 83924-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Front Nutr

Luteolin attenuates LPS-induced damage in IPEC-J2 cells by enhancing mitophagy via AMPK signaling pathway activation

Authors - Jianyun Yuan
humanWB

Anal Cell Pathol (Amst)

Shikonin Induces Autophagy and Apoptosis in Esophageal Cancer EC9706 Cells by Regulating the AMPK/mTOR/ULK Axis

Authors - Junli Zhang
humanWB

Cell Signal

Targeted therapy for KIF3C: A study on the mechanism of combined therapy with KIF3C signaling pathway, afatinib, and MT-DC (ac)phosphoramide in regulating gastric cancer cell proliferation

Authors - Maladho Tanta Diallo
mouseWB

Nutrients

Protective Effects of Isostrictiniin Against High-Fat, High-Sugar Diet-Induced Steatosis in MASLD Mice via Regulation of the AMPK/SREBP-1c/ACC Pathway

Authors - Qi Yan
mouseWB

Biomaterials

Selective and iron-independent ferroptosis in cancer cells induced by manipulation of mitochondrial fatty acid oxidation

Authors - Yan Gao
mouseWB

Diabetes Metab J

Shionone Inhibits Glomerular Fibirosis by Suppressing NLRP3 Related Inflammasome though SESN2-NRF2/ HO-1 Pathway

Authors - Tian Xiao
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