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Phospho-AKT (Ser473) Monoclonal antibody, PBS Only

Phospho-AKT (Ser473) Monoclonal Antibody for WB,IHC,FC,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB,IHC,FC,ELISA

Conjugate

Unconjugated

CloneNo.

1C10B8

Cat No : 66444-1-PBS

Print datasheet

Synonyms

AKT, AKT1, pakt, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha

验证数据展示

  • WB analysis using 66444-1-Ig (same clone as 66444-1-PBS)

    WB analysis using 66444-1-Ig (same clone as 66444-1-PBS)

    Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 66444-1-Ig (Phospho-AKT (Ser473) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 66444-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66444-1-Ig (same clone as 66444-1-PBS)

    IHC staining of human breast cancer using 66444-1-Ig (same clone as 66444-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66444-1-Ig (AKT-phospho-S473 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66444-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AKT antibody included
  • IHC staining of human breast cancer using 66444-1-Ig (same clone as 66444-1-PBS)

    IHC staining of human breast cancer using 66444-1-Ig (same clone as 66444-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66444-1-Ig (AKT-phospho-S473 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66444-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AKT antibody included
  • FC experiment of PC-3 using 66444-1-Ig (same clone as 66444-1-PBS)

    FC experiment of PC-3 using 66444-1-Ig (same clone as 66444-1-PBS)

    1X10^6 PC-3 cells untreated (dashed line) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-AKT (Ser473) (66444-1-Ig, Clone:1C10B8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 66444-1-PBS in a different storage buffer formulation.

  • IHC staining of Jurkat using 66444-1-Ig (same clone as 66444-1-PBS)

    IHC staining of Jurkat using 66444-1-Ig (same clone as 66444-1-PBS)

    Immunohistochemical analysis of paraffin-embedded untreated (left) or Calyculin A treated (right) Jurkat cells slide using 66444-1-Ig (Phospho-AKT (Ser473) antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66444-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AKT antibody included
  • IHC staining of Jurkat using 66444-1-Ig (same clone as 66444-1-PBS)

    IHC staining of Jurkat using 66444-1-Ig (same clone as 66444-1-PBS)

    Immunohistochemical analysis of paraffin-embedded untreated (left) or Calyculin A treated (right) Jurkat cells slide using 66444-1-Ig (Phospho-AKT (Ser473) antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66444-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AKT antibody included
Planning an IHC experiment? We recommend our IHCeasy AKT Ready-To-Use IHC Kit. AKT primary antibody included.

产品信息

66444-1-PBS targets Phospho-AKT (Ser473) in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB,IHC,FC,ELISA
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name v-akt murine thymoma viral oncogene homolog 1
Synonyms AKT, AKT1, pakt, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha
Observed Molecular Weight 60-62 kDa
GenBank Accession NumberNM_005163
Gene Symbol AKT1
Gene ID (NCBI) 207
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP31749
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with phospho-modification at Ser473.