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验证数据展示

  • WB analysis using 33132-1-AP (same clone as 33132-1-PBS)

    WB analysis using 33132-1-AP (same clone as 33132-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33132-1-AP (PDC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33132-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse eye using 33132-1-AP (same clone as 33132-1-PBS)

    IHC staining of mouse eye using 33132-1-AP (same clone as 33132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 33132-1-AP (Phosducin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33132-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse eye using 33132-1-AP (same clone as 33132-1-PBS)

    IHC staining of mouse eye using 33132-1-AP (same clone as 33132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 33132-1-AP (Phosducin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33132-1-PBS in a different storage buffer formulation.

Phosducin Polyclonal antibody, PBS Only

Phosducin Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 33132-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

33 kDa phototransducing protein, PDC, PHD, Protein MEKA

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

33132-1-PBS targets Phosducin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag37137

Product name: Recombinant human PDC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 31-140 aa of NM_002597.4

Sequence: KFKLESQDSDSIPPSKKEILRQMSSPQSRNGKDSKERVSRKMSIQEYELIHKEKEDENCLRKYRRQCMQDMHQKLSFGPRYGFVYELETGKQFLETIEKELKITTIVVHI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosducin
别名 33 kDa phototransducing protein, PDC, PHD, Protein MEKA
计算分子量28 kDa, 246 aa
观测分子量32 kDa
GenBank蛋白编号NM_002597.4
基因名称 PDC
Gene ID (NCBI) 5132
RRIDAB_3742864
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP20941
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Phosducin/PDC, which tightly binds betagamma-subunits of heterotrimeric G-proteins, has been conjectured to play a role in regulating second messenger signaling cascades. Phosducin may act as a phosphorylation-dependent switch in second messenger signaling cascades, regulating the kinetics of desensitization processes by controlling the activity of Gbetagamma-dependent GRKs (PMID: 9020189).

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