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验证数据展示

  • IF Staining of HeLa using 15294-1-AP (same clone as 15294-1-PBS)

    IF Staining of HeLa using 15294-1-AP (same clone as 15294-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using Perilipin-2 antibody (15294-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

  • WB analysis using 15294-1-AP (same clone as 15294-1-PBS)

    WB analysis using 15294-1-AP (same clone as 15294-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15294-1-AP (Perilipin-2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 15294-1-AP (same clone as 15294-1-PBS)

    IHC staining of human liver cancer using 15294-1-AP (same clone as 15294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ADRP antibody included
  • IHC staining of human liver cancer using 15294-1-AP (same clone as 15294-1-PBS)

    IHC staining of human liver cancer using 15294-1-AP (same clone as 15294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ADRP antibody included
  • IF Staining of HeLa using 15294-1-AP (same clone as 15294-1-PBS)

    IF Staining of HeLa using 15294-1-AP (same clone as 15294-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP/Perilipin 2 antibody (15294-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 15294-1-AP (same clone as 15294-1-PBS)

    IF Staining of HeLa using 15294-1-AP (same clone as 15294-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP/Perilipin-2 antibody (15294-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Perilipin-2 Polyclonal antibody, PBS Only

Perilipin-2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15294-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ADFP, Adipophilin, Adipose differentiation-related protein, ADRP, ADRP/Perilipin 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ADRP Ready-To-Use IHC Kit. ADRP primary antibody included.

产品信息

15294-1-PBS targets Perilipin-2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7539

Product name: Recombinant human ADRP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 88-437 aa of BC005127

Sequence: DRIEERLPILNQPSTQIVANAKGAVTGAKDAVTTTVTGAKDSVASTITGVMDKTKGAVTGSVEKTKSVVSGSINTVLGSRMMQLVSSGVENALTKSELLVEQYLPLTEEELEKEAKKVEGFDLVQKPSYYVRLGSLSTKLHSRAYQQALSRVKEAKQKSQQTISQLHSTVHLIEFARKNVYSANQKIQDAQDKLYLSWVEWKRSIGYDDTDESHCAEHIESRTLAIARNLTQQLQTTCHTLLSNIQGVPQNIQDQAKHMGVMAGDIYSVFRNAASFKEVSDSLLTSSKGQLQKMKESLDDVMDYLVNNTPLNWLVGPFYPQLTESQNAQDQGAEMDKSSQETQRSEHKTH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adipose differentiation-related protein
别名 ADFP, Adipophilin, Adipose differentiation-related protein, ADRP, ADRP/Perilipin 2
计算分子量 48 kDa
观测分子量 45-48 kDa
GenBank蛋白编号BC005127
基因名称 Perilipin-2
Gene ID (NCBI) 123
RRIDAB_2878122
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99541
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases. This antibody is not suitable for immunofluorescence staining of frozen sections.

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