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验证数据展示

  • WB analysis using 66674-4-Ig (same clone as 66674-4-PBS)

    WB analysis using 66674-4-Ig (same clone as 66674-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66674-4-Ig (PARK2/Parkin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66674-4-PBS in a different storage buffer formulation.

  • WB analysis using 66674-4-Ig (same clone as 66674-4-PBS)

    WB analysis using 66674-4-Ig (same clone as 66674-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66674-4-Ig (PARK2/Parkin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66674-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66674-4-Ig (same clone as 66674-4-PBS)

    IHC staining of mouse brain using 66674-4-Ig (same clone as 66674-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66674-4-Ig (PARK2/Parkin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66674-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PARK2 antibody included
  • IF Staining of mouse brain using 66674-4-Ig (same clone as 66674-4-PBS)

    IF Staining of mouse brain using 66674-4-Ig (same clone as 66674-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PARK2/Parkin antibody (66674-4-Ig, Clone: 2G4A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66674-4-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 66674-4-Ig (same clone as 66674-4-PBS)

    IF Staining of mouse brain using 66674-4-Ig (same clone as 66674-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PARK2/Parkin antibody (66674-4-Ig, Clone: 2G4A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66674-4-PBS in a different storage buffer formulation.

PARK2/Parkin Monoclonal antibody, PBS Only

PARK2/Parkin Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66674-4-PBS

产品说明书

CloneNo. 2G4A8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, Indirect ELISA

别名

PARK2, Parkin, E3 ubiquitin-protein ligase parkin, EC:2.3.2.31, Parkin RBR E3 ubiquitin-protein ligase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PARK2 Ready-To-Use IHC Kit. PARK2 primary antibody included.

产品信息

66674-4-PBS targets PARK2/Parkin in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag5179

Product name: Recombinant human Parkin protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 81-387 aa of BC022014

Sequence: NATGGDDPRNAAGGCEREPQSLTRVDLSSSVLPGDSVGLAVILHTDSRKDSPPAGSPAGRSIYNSFYVYCKGPCQRVQPGKLRVQCSTCRQATLTLTQGPSCWDDVLIPNRMSGECQSPHCPGTSAEFFFKCGAHPTSDKETSVALHLIATNSRNITCITCTDVRSPVLVFQCNSRHVICLDCFHLYCVTRLNDRQFVHDPQLGYSLPCVGTGDTVVLRGALGGFRRGVAGCPNSLIKELHHFRILGEEQYNRYQQYGAEECVLQMGGVLCPRPGCGAGLLPEPDQRKVTCEGGNGLGCGYGQRRTK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Parkinson disease (autosomal recessive, juvenile) 2, parkin
别名 PARK2, Parkin, E3 ubiquitin-protein ligase parkin, EC:2.3.2.31, Parkin RBR E3 ubiquitin-protein ligase
计算分子量 52 kDa
观测分子量 42-52 kDa
GenBank蛋白编号BC022014
基因名称 Parkin
Gene ID (NCBI) 5071
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO60260
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a viariety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing.

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