验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, C6 cells, HUVEC cells, HeLa cells, MCF-7 cells, mouse brain tissue, mouse heart tissue, human placenta tissue |
| Positive IHC detected in | mouse colon tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
31313-1-AP targets Pan-cadherin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cadherin 1, type 1, E-cadherin (epithelial) |
| 别名 | Pan Cadherin, E-cadherin, Arc 1, Cadherin 1, Cadherin-1 |
| 计算分子量 | 97 kDa |
| 观测分子量 | 120-130 kDa |
| GenBank蛋白编号 | NM_004360 |
| 基因名称 | E-cadherin |
| Gene ID (NCBI) | 999 |
| RRID | AB_3669943 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P12830 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in tissue morphogenesis and the maintenance of normal tissue architecture (PMID: 23885883). The classic cadherin family members include E-, N-, P-, R-, and B-cadherins. The classic cadherins have ectodomains composed of five extracellular cadherin (EC) repeats, a single transmembrane region, and a cytoplasmic domain. Structural characterization indicated that the known cadherins are glycoproteins ranging in their apparent molecular weights between 120 and 140 kDa (PMID: 2077036). This polyclonal antibody is raised against a synthetic peptide corresponding to a conserved region of human E-cadherin and can detect E-, N-, P-, VE-, and R-cadherins.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Pan-cadherin antibody 31313-1-AP | Download protocol |
| IHC protocol for Pan-cadherin antibody 31313-1-AP | Download protocol |
| WB protocol for Pan-cadherin antibody 31313-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Med circFOXK2 Stabilizes STMN1 mRNA via PABPC1 to Promote the Progression of NSCLC | ||
Biomaterials Radiation-triggerable bioreactors enable bioenergetic reprograming of cancer stem cell plasticity via targeted arginine metabolism disruption for augmented radio-immunotherapy |
