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验证数据展示

  • WB analysis of HeLa using 84289-7-RR

    WB analysis of HeLa using 84289-7-RR

    WB result of PYROXD1 antibody (84289-7-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYROXD1 transfected HeLa cells.

  • WB analysis using 84289-7-RR

    WB analysis using 84289-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84289-7-RR (PYROXD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84289-7-RR

    Affinity and Kinetic Characterization of 84289-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84289-7-RR against Human PYROXD1 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

PYROXD1 Recombinant antibody

PYROXD1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84289-7-RR

产品说明书

CloneNo. 241639D7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

241639D7, pyridine nucleotide disulphide oxidoreductase domain 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, U-87 MG cells, Caco-2 cells, COLO 320 cells, PC-3 cells, THP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84289-7-RR targets PYROXD1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 PYROXD1 fusion protein Ag34768 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pyridine nucleotide-disulphide oxidoreductase domain 1
别名 241639D7, pyridine nucleotide disulphide oxidoreductase domain 1
观测分子量55~60 kDa
GenBank蛋白编号BC021662
基因名称 PYROXD1
Gene ID (NCBI) 79912
RRIDAB_3671834
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8WU10
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

pyridine nucleotide-disulfide oxidoreductase domain 1 (PYROXD1) is a putative oxidoreductase that co-evolved with the tRNA-LC and is essential for its activity in cells and in vivo. PYROXD1 oxidizes NAD(P)H to NAD(P)+ with tightly controlled kinetics, reducing one molecule of O2 into H2O2 per turnover. PYROXD1 is expressed across a range of tissue types in humans, including skeletal muscle, and is conserved across many species.

实验方案

Product Specific Protocols
WB protocol for PYROXD1 antibody 84289-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols