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验证数据展示

  • WB analysis of HeLa using 31093-1-AP

    WB analysis of HeLa using 31093-1-AP

    WB result of PYROXD1 antibody (31093-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYROXD1 transfected HeLa cells.

  • WB analysis using 31093-1-AP

    WB analysis using 31093-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31093-1-AP (PYROXD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

PYROXD1 Polyclonal antibody

PYROXD1 Polyclonal Antibody for WB, ELISA
Cat No. 31093-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

pyridine nucleotide disulphide oxidoreductase domain 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells, HeLa cells, U-87 MG cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31093-1-AP targets PYROXD1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 PYROXD1 fusion protein Ag34768 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pyridine nucleotide-disulphide oxidoreductase domain 1
别名 pyridine nucleotide disulphide oxidoreductase domain 1
观测分子量55 kDa
GenBank蛋白编号BC021662
基因名称 PYROXD1
Gene ID (NCBI) 79912
RRIDAB_3669851
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WU10
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

pyridine nucleotide-disulfide oxidoreductase domain 1 (PYROXD1) is a putative oxidoreductase that co-evolved with the tRNA-LC and is essential for its activity in cells and in vivo. PYROXD1 oxidizes NAD(P)H to NAD(P)+ with tightly controlled kinetics, reducing one molecule of O2 into H2O2 per turnover. PYROXD1 is expressed across a range of tissue types in humans, including skeletal muscle, and is conserved across many species.

实验方案

Product Specific Protocols
WB protocol for PYROXD1 antibody 31093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols