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验证数据展示

  • WB analysis of MDA-MB-453s using 11555-1-AP (same clone as 11555-1-PBS)

    WB analysis of MDA-MB-453s using 11555-1-AP (same clone as 11555-1-PBS)

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11555-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 11555-1-AP (same clone as 11555-1-PBS)

    IHC staining of human lung cancer using 11555-1-AP (same clone as 11555-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11555-1-AP (PYGO2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11555-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PYGO2 Polyclonal antibody, PBS Only

PYGO2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11555-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

PYGO 2, PP7910

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11555-1-PBS targets PYGO2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2111

Product name: Recombinant human PYGO2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 101-406 aa of BC032099

Sequence: FGGFRVQGGMAGQVPPGYSTGGGGGPQPLRRQPPPFPPNPMGPAFNMPPQGPGYPPPGNMNFPSQPFNQPLGQNFSPPSGQMMPGPVGGFGPMISPTMGQPPRAELGPPSLSQRFAQPGAPFGPSPLQRPGQGLPSLPPNTSPFPGPDPGFPGPGGEDGGKPLNPPASTAFPQEPHSGSPAAAVNGNQPSFPPNSSGRGGGTPDANSLAPPGKAGGGSGPQPPPGLVYPCGACRSEVNDDQDAILCEASCQKWFHRECTGMTESAYGLLTTEASAVWACDLCLKTKEIQSVYIREGMGQLVAANDG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pygopus homolog 2 (Drosophila)
别名 PYGO 2, PP7910
计算分子量 406 aa, 41 kDa
观测分子量 50 kDa
GenBank蛋白编号BC032099
基因名称 PYGO2
Gene ID (NCBI) 90780
RRIDAB_2877776
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BRQ0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PYGO2, also named as Pygopus homolog 2, is a 406 animo acid protein, which contains 1 PHD-type zinc finger. PYGO2 is involved in signal transduction through the Wnt pathway and Binds to BCL9 via the PHD-type zinc finger motif, and thereby becomes part of the nuclear beta-catenin/TCF complex. PYGO2 is in a high proportion of breast and epithelial ovarian malignant tumors and is required for the growth of several cell lines derived from these carcinomas.

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