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验证数据展示

  • WB analysis using 16008-1-AP (same clone as 16008-1-PBS)

    WB analysis using 16008-1-AP (same clone as 16008-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16008-1-AP (PYCRL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16008-1-PBS in a different storage buffer formulation.

  • WB analysis using 16008-1-AP (same clone as 16008-1-PBS)

    WB analysis using 16008-1-AP (same clone as 16008-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16008-1-AP (PYCRL antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16008-1-PBS in a different storage buffer formulation.

PYCRL Polyclonal antibody, PBS Only

PYCRL Polyclonal Antibody for WB, Indirect ELISA
Cat No. 16008-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

PYCR3, EC:1.5.1.2, P5C reductase 3, P5CR 3, Pyrroline-5-carboxylate reductase 3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16008-1-PBS targets PYCRL in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag8716

Product name: Recombinant human PYCRL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-274 aa of BC007993

Sequence: MAAAEPSPRRVGFVGAGRMAGAIAQGLIRAGKVEAQHILASAPTDRNLCHFQALGCRTTHSNQEVLQSCLLVIFATKPHVLPAVLAEVAPVVTTEHILVSVAAGVSLSTLEELLPPNTRVLRVLPNLPCVVQEGAIVMARGRHVGSSETNLLQHLLEACGRCEEVPEAYVDIHTGLSGSGVAFVCAFSEALAEGAVKMGMPSSLAHRIAAQTLLGTAKMLLHEGQHPAQLRSDVCTPGGTTIYGLHALEQGGLRAATMSAVEAATCRAKELSRK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pyrroline-5-carboxylate reductase-like
别名 PYCR3, EC:1.5.1.2, P5C reductase 3, P5CR 3, Pyrroline-5-carboxylate reductase 3
计算分子量 274 aa, 29 kDa
观测分子量29 kDa
GenBank蛋白编号BC007993
基因名称 PYCRL
Gene ID (NCBI) 65263
RRIDAB_3669228
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ53H96
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Proline conversion into P5C is coupled to the P5C reduction into proline, catalyzed by P5C reductase. This enzyme has three isoforms: PYCR1/PYCR2, which are specific for the mitochondrial compartment and the PYCRL present in cytosol. The conversion of P5C to proline, and its transport between mitochondria and cytosol, are also coupled with glucose metabolism by the pentose phosphate pathway.(PMID: 35163433)

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