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PYCR2-specific Polyclonal antibody

PYCR2-specific Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 55060-1-AP

Print datasheet

Synonyms

P5C reductase 2, P5CR 2, P5CR2, PYCR2

验证数据展示

  • WB analysis of HeLa using 55060-1-AP

    WB analysis of HeLa using 55060-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55060-1-AP (PYCR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 55060-1-AP

    IF Staining of HeLa using 55060-1-AP

    Immunofluorescent analysis of HeLa cells using 55060-1-AP (PYCR2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

经过测试的应用

Positive WB detected inHeLa cells, L02 cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55060-1-AP targets PYCR2-specific in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pyrroline-5-carboxylate reductase family, member 2
Synonyms P5C reductase 2, P5CR 2, P5CR2, PYCR2
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 27-31 kDa, 20 kDa
GenBank Accession NumberNM_013328
Gene Symbol PYCR2
Gene ID (NCBI) 29920
RRIDAB_10888635
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96C36
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody is specific to PYCR2 and has no cross reaction to PYCR1.

实验方案

Product Specific Protocols
WB protocol for PYCR2-specific antibody 55060-1-APDownload protocol
IF protocol for PYCR2-specific antibody 55060-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Cell Rep

Proline restores mitochondrial function and reverses aging hallmarks in senescent cells

Authors - Debanik Choudhury
View Article
humanWB

Sci Rep

Activation of proline biosynthesis is critical to maintain glutamate homeostasis during acute methamphetamine exposure.

Authors - Bobby Jones
View Article