验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16315-1-AP targets PXK in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | PXK fusion protein Ag9362 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | PX domain containing serine/threonine kinase |
| 别名 | PX domain-containing protein kinase-like protein, MONaKA, Modulator of Na,K-ATPase, Modulator of Na,K ATPase |
| 计算分子量 | 450aa,52 kDa; 578aa,65 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC014479 |
| 基因名称 | PXK |
| Gene ID (NCBI) | 54899 |
| RRID | AB_3669237 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z7A4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PXK is part of the sorting nexin (SNX) family of proteins, which are important for receptor internalization, organelle trafficking including endosomal trafficking, and other membrane-centric sorting functions. This is accomplished primarily through the PX-domain mediated binding of PI3P . PXK was first identified and cloned by two independent groups in 2005. Initial studies established that PXK is detectable in most tissues with a primarily cytoplasmic distribution. (PMID: 25620976)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PXK antibody 16315-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |