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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 32112-1-AP

    WB analysis using 32112-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32112-1-AP (PWWP2B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

PWWP2B Polyclonal antibody

PWWP2B Polyclonal Antibody for WB, ELISA
Cat No. 32112-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

PWWP Domain Containing 2, PWWP Domain Containing 2B , PWWP domain-containing protein 2B, PWWP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, BxPC-3 cells, HaCaT cells, HeLa cells, LNCaP cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32112-1-AP targets PWWP2B in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag36839

Product name: Recombinant human PWWP2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 199-413 aa of BC068574

Sequence: SLEPFRPQQAPQDDGSQDPEVLDRESRDRPSCAPSASIPKLKLTRPVPAGADLPPPKIRLKPHRLGDSEHEPVYRAELVGELNGYLRDSSPAPCADGPAGGLADLSSGSSGEDDDFKSCPQGPQGREGLAFLVSCPEGRADCASESACSSDSLDEARSSGSEGTPADTGDLSPGHGASAPSVSREARQTVPPLTVRLHTQSVSECITEDGRTVAV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PWWP domain containing 2B
别名 PWWP Domain Containing 2, PWWP Domain Containing 2B , PWWP domain-containing protein 2B, PWWP2
观测分子量90 kDa
GenBank蛋白编号BC068574
基因名称 PWWP2B
Gene ID (NCBI) 170394
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ6NUJ5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PWWP2B (PWWP structural domain protein 2B) is a PWWP structural domain-containing protein involved in a variety of cellular functions and biological processes. PWWP2B is highly expressed in brown and beige adipose tissue and its expression is induced by cold stimulation. It has been shown that PWWP2B negatively regulates adipose tissue thermogenesis by recruiting and stabilizing the histone deacetylase HDAC1/2, which binds to the promoter regions of thermogenic genes and reduces histone acetylation levels. In adipocytes, PWWP2B acts as part of the nucleosome remodeling and deacetylation complex (NuRD) to inhibit the expression of thermogenic genes and avoid overactivation of thermogenic functions. In addition, PWWP2B plays an important role in DNA damage repair, as its PWWP structural domain moves to DNA damage sites, interacts with UHRF1 and recruits MRE11 to promote DNA end resection and homologous recombination repair. In pathology, downregulation of PWWP2B is associated with recurrent gastric cancer and promotes cell proliferation and migration.

实验方案

Product Specific Protocols
WB protocol for PWWP2B antibody 32112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols