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验证数据展示

  • WB analysis of HeLa using 11390-1-AP

    WB analysis of HeLa using 11390-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11390-1-AP (PWP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 11390-1-AP

    IF Staining of HepG2 using 11390-1-AP

    Immunofluorescent analysis of HepG2 cells, using PWP2 antibody 11390-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of HeLa using 11390-1-AP

    IP experiment of HeLa using 11390-1-AP

    IP result of anti-PWP2 (IP:11390-1-AP, 4ug; Detection:11390-1-AP 1:600) with HeLa cells lysate 2400ug.

PWP2 Polyclonal antibody

PWP2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 11390-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

PWP2H

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11390-1-AP targets PWP2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag1936

Product name: Recombinant human PWP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 520-919 aa of BC014988

Sequence: SWDKTVRLWDMFDSWRTKETLALTSDALAVTIRPDGAELAVATLNSQITFWDPENAVQTGSIEGRHDLKTGRKELDKITAKHAAKGKAFTALCYSADGHSILAGGMSKFVCIYHVREQILMKRFEISCNLSLDAMEEFLNRRKMTEFGNLALIDQDAGQEDGVAIPLPGVRKGDMSSRHFKPEIRVTSLRFSPTGRCWAATTTEGLLIYSLDTRVLFDPFELDTSVTPGRVREALRQQDFTRAILMALRLNESKLVQEALEAVPRGEIEVVTSSLPELYVEKVLEFLASSFEVSRHLEFYLLWTHKLLMLHGQKLKSRAGTLLPVIQFLQKSIQRHLDDLSKLCSWNHYNMQYALAVSKQRGTKRSLDPLGSEEEAEASEDDSLHLLGGGGRDSEEEMLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PWP2 periodic tryptophan protein homolog (yeast)
别名 PWP2H
计算分子量 919 aa, 102 kDa
观测分子量 102 kDa
GenBank蛋白编号BC014988
基因名称 PWP2
Gene ID (NCBI) 5822
RRIDAB_2284709
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15269
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The function of PWP2 remains largely unknown. Catalog#11390-1-AP is a rabbit polyclonal antibody raised against the full-length of human PWP2. The MW of this protein is 102 kDa, and this antibody specially recognises the 102 kDa protein.

实验方案

Product Specific Protocols
WB protocol for PWP2 antibody 11390-1-APDownload protocol
IF protocol for PWP2 antibody 11390-1-APDownload protocol
IP protocol for PWP2 antibody 11390-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

Derivation and Validation of a Prognostic Model for Cancer Dependency Genes Based on CRISPR-Cas9 in Gastric Adenocarcinoma.

Authors - Wenjie Zhou
View Article