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PWP2 Polyclonal antibody

PWP2 Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 11390-1-AP

Print datasheet

Synonyms

EHOC 17, PWP2, PWP2H

验证数据展示

  • WB analysis of HeLa using 11390-1-AP

    WB analysis of HeLa using 11390-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11390-1-AP (PWP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 11390-1-AP

    IF Staining of HepG2 using 11390-1-AP

    Immunofluorescent analysis of HepG2 cells, using PWP2 antibody 11390-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of HeLa using 11390-1-AP

    IP experiment of HeLa using 11390-1-AP

    IP result of anti-PWP2 (IP:11390-1-AP, 4ug; Detection:11390-1-AP 1:600) with HeLa cells lysate 2400ug.

经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11390-1-AP targets PWP2 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen PWP2 fusion protein Ag1936 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PWP2 periodic tryptophan protein homolog (yeast)
Synonyms EHOC 17, PWP2, PWP2H
Calculated Molecular Weight 919 aa, 102 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC014988
Gene Symbol PWP2
Gene ID (NCBI) 5822
RRIDAB_2284709
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15269
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The function of PWP2 remains largely unknown. Catalog#11390-1-AP is a rabbit polyclonal antibody raised against the full-length of human PWP2. The MW of this protein is 102 kDa, and this antibody specially recognises the 102 kDa protein.

实验方案

Product Specific Protocols
WB protocol for PWP2 antibody 11390-1-APDownload protocol
IF protocol for PWP2 antibody 11390-1-APDownload protocol
IP protocol for PWP2 antibody 11390-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

Derivation and Validation of a Prognostic Model for Cancer Dependency Genes Based on CRISPR-Cas9 in Gastric Adenocarcinoma.

Authors - Wenjie Zhou
View Article