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验证数据展示

  • WB analysis of A549 using 17248-1-AP

    WB analysis of A549 using 17248-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 17248-1-AP (PUS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 17248-1-AP

    WB analysis using 17248-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17248-1-AP (PUS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

PUS3 Polyclonal antibody

PUS3 Polyclonal Antibody for WB, ELISA
Cat No. 17248-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA and More (1)

别名

2610020J05Rik, FKSG32, pseudouridylate synthase 3, PUS3, tRNA pseudouridine synthase 3, tRNA uridine isomerase 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17248-1-AP targets PUS3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag9983

Product name: Recombinant human PUS3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 140-482 aa of BC004822

Sequence: RDSEDFNVKEEANAAAEEIRYTHILNRVLPPDIRILAWAPVEPSFSARFSCLERTYRYFFPRADLDIVTMDYAAQKYVGTHDFRNLCKMDVANGVINFQRTILSAQVQLVGQSPGEGRWQEPFQLCQFEVTGQAFLYHQVRCMMAILFLIGQGMEKPEIIDELLNIEKNPQKPQYSMAVEFPLVLYDCKFENVKWIYDQEAQEFNITHLQQLWANHAVKTHMLYSMLQGLDTVPVPCGIGPKMDGMTEWGNVKPSVIKQTSAFVEGVKMRTYKPLMDRPKCQGLESRIQHFVRRGRIEHPHLFHEEETKAKRDCNDTLEEDNTNLETPTKRVCVDTEIKSII

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pseudouridylate synthase 3
别名 2610020J05Rik, FKSG32, pseudouridylate synthase 3, PUS3, tRNA pseudouridine synthase 3, tRNA uridine isomerase 3
计算分子量 56 kDa
观测分子量 50-52 kDa
GenBank蛋白编号BC004822
基因名称 PUS3
Gene ID (NCBI) 83480
RRIDAB_2237874
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BZE2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PUS3 antibody 17248-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

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Targeted Quantitative Profiling of Epitranscriptomic Reader, Writer, and Eraser Proteins Using Stable Isotope-Labeled Peptides

Authors - Tianyu F Qi
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