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验证数据展示

  • WB analysis of rat brain using 86129-1-RR

    WB analysis of rat brain using 86129-1-RR

    rat brain tissue were subjected to SDS PAGE followed by western blot with 86129-1-RR (PUM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86129-1-RR

    WB analysis using 86129-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86129-1-RR (PUM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 86129-1-RR

    IF Staining of HeLa using 86129-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PUM1 antibody (86129-1-RR, Clone: 250792G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PUM1 Recombinant antibody

PUM1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86129-1-RR

产品说明书

CloneNo. 250792G4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

HsPUM, KIAA0099, PUMH, PUMH1, Pumilio 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, rat brain tissue, A549 cells, K-562 cells, HEK-293 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PUM1 Ready-To-Use IHC Kit. PUM1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86129-1-RR targets PUM1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PUM1 fusion protein Ag24651 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pumilio homolog 1 (Drosophila)
别名 HsPUM, KIAA0099, PUMH, PUMH1, Pumilio 1
计算分子量 1186 aa, 126 kDa
观测分子量140 kDa
GenBank蛋白编号BC013398
基因名称 PUM1
Gene ID (NCBI) 9698
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14671
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PUM1, also named as Pumilio homolog 1, is a 1186 amino acid protein, which is expressed in brain, heart, kidney, muscle, intestine and stomach. PUM1 as sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. PUM1 binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). The calculated molecular weight of PUM1 is 126 kDa, but the modified PUM1 is about 130-140 kDa.

实验方案

Product Specific Protocols
WB protocol for PUM1 antibody 86129-1-RRDownload protocol
IF protocol for PUM1 antibody 86129-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols