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验证数据展示

  • WB analysis using 86343-2-RR

    WB analysis using 86343-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86343-2-RR (PTRH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86343-2-RR

    Affinity and Kinetic Characterization of 86343-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86343-2-RR against Human PTRH2 were performed. The affinity constant is below 1 pM.

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PTRH2 Recombinant monoclonal antibody

PTRH2 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86343-2-RR

产品说明书

CloneNo. 251056C4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Bcl-2 inhibitor of transcription 1, BIT1, CGI 147, CGI-147, EC:3.1.1.29

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, Raji cells, MCF-7 cells, Jurkat cells, A549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86343-2-RR targets PTRH2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0839

Product name: Recombinant human PTRH2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-180 aa of BC006807

Sequence: MPSKSLVMEYLAHPSTLGLAVGVACGMCLGWSLRVCFGMLPKSKTSKTHTDTESEASILGDSGEYKMILVVRNDLKMGKGKVAAQCSHAAVSAYKQIQRRNPEMLKQWEYCGQPKVVVKAPDEETLIALLAHAKMLGLTVSLIQDAGRTQIAPGSQTVLGIGPGPADLIDKVTGHLKLY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 peptidyl-tRNA hydrolase 2
别名 Bcl-2 inhibitor of transcription 1, BIT1, CGI 147, CGI-147, EC:3.1.1.29
计算分子量 19 kDa
观测分子量 19 kDa
GenBank蛋白编号BC006807
基因名称 PTRH2
Gene ID (NCBI) 51651
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y3E5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTRH2 (Peptidyl-tRNA Hydrolase 2, also known as Bcl-2 Inhibitor of Transcription 1 or BIT1) is a highly conserved protein that plays crucial roles in various cellular processes, including protein synthesis, cell survival, and cell death.

实验方案

Product Specific Protocols
WB protocol for PTRH2 antibody 86343-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols