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验证数据展示

  • WB analysis using 51006-2-AP

    WB analysis using 51006-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 51006-2-AP (PTRH2 antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.

  • IHC staining of human prostate cancer using 51006-2-AP

    IHC staining of human prostate cancer using 51006-2-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 51006-2-AP (PTRH2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 51006-2-AP

    IHC staining of human prostate cancer using 51006-2-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 51006-2-AP (PTRH2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 51006-2-AP

    IF Staining of HEK-293 using 51006-2-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PTRH2 antibody (51006-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

PTRH2 Polyclonal antibody

PTRH2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 51006-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Bcl-2 inhibitor of transcription 1, BIT1, CGI 147, CGI-147, EC:3.1.1.29

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, Jurkat cells, MCF-7 cells, Raji cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51006-2-AP targets PTRH2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0839

Product name: Recombinant human PTRH2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-180 aa of BC006807

Sequence: MPSKSLVMEYLAHPSTLGLAVGVACGMCLGWSLRVCFGMLPKSKTSKTHTDTESEASILGDSGEYKMILVVRNDLKMGKGKVAAQCSHAAVSAYKQIQRRNPEMLKQWEYCGQPKVVVKAPDEETLIALLAHAKMLGLTVSLIQDAGRTQIAPGSQTVLGIGPGPADLIDKVTGHLKLY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidyl-tRNA hydrolase 2
别名 Bcl-2 inhibitor of transcription 1, BIT1, CGI 147, CGI-147, EC:3.1.1.29
计算分子量 19 kDa
观测分子量 19 kDa
GenBank蛋白编号BC006807
基因名称 PTRH2
Gene ID (NCBI) 51651
RRIDAB_2173280
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y3E5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTRH2 (Peptidyl-tRNA Hydrolase 2, also known as Bcl-2 Inhibitor of Transcription 1 or BIT1) is a highly conserved protein that plays crucial roles in various cellular processes, including protein synthesis, cell survival, and cell death.

实验方案

Product Specific Protocols
IF protocol for PTRH2 antibody 51006-2-APDownload protocol
IHC protocol for PTRH2 antibody 51006-2-APDownload protocol
WB protocol for PTRH2 antibody 51006-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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