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验证数据展示

  • WB analysis using 17651-1-AP

    WB analysis using 17651-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17651-1-AP (PTRH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PTRH1 Polyclonal antibody

PTRH1 Polyclonal Antibody for WB, ELISA
Cat No. 17651-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

C9orf115, EC:3.1.1.29, Peptidyl-tRNA hydrolase, PTH, PTH1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells, Caco-2 cells, HeLa cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17651-1-AP targets PTRH1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag11893

Product name: Recombinant human PTRH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-214 aa of BC047012

Sequence: MRPGGFLGAGQRLSRAMSRCVLEPRPPGKRWMVAGLGNPGLPGTRHSVGMAVLGQLARRLGVAESWTRDRHCAADLALAPLGDAQLVLLRPRRLMNANGRSVARAAELFGLTAEEVYLVHDELDKPLGRLALKLGGSARGHNGVRSCISCLNSNAMPRLRVGIGRPAHPEAVQAHVLGCFSPAEQELLPLLLDRATDLILDHIRERSQGPSLGP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidyl-tRNA hydrolase 1 homolog (S. cerevisiae)
别名 C9orf115, EC:3.1.1.29, Peptidyl-tRNA hydrolase, PTH, PTH1
计算分子量 214 aa, 23 kDa
观测分子量 23 kDa
GenBank蛋白编号BC047012
基因名称 PTRH1
Gene ID (NCBI) 138428
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86Y79
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peptidyl-tRNA hydrolase 1 homolog(PTRH1), also known as PTH1, is peptidyl-tRNA hydrolase that cleaves nascent chains-tRNAs that are not stably fixed in the P-site of 60S ribosome-nascent chain complexes. PTRH1 functions in the ribosome-associated quality control (RQC) pathway and is involved in the degradation of nonubiquitinated nascent chain (NC) polypeptides arising from interrupted translation(PMID:30244831).

实验方案

Product Specific Protocols
WB protocol for PTRH1 antibody 17651-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols