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验证数据展示

  • WB analysis using 30086-1-AP

    WB analysis using 30086-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30086-1-AP (PTRF antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 30086-1-AP

    IHC staining of human stomach using 30086-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 30086-1-AP (PTRF antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTRF antibody included
  • IF Staining of HepG2 using 30086-1-AP

    IF Staining of HepG2 using 30086-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PTRF antibody (30086-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PTRF Polyclonal antibody

PTRF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30086-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

FKSG13, Cavin-1, Cavin 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HT-1080 cells, NIH/3T3 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PTRF Ready-To-Use IHC Kit. PTRF primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30086-1-AP targets PTRF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PTRF fusion protein Ag32786 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase I and transcript release factor
别名 FKSG13, Cavin-1, Cavin 1
计算分子量 43 kDa
观测分子量55 kDa
GenBank蛋白编号BC008849
基因名称 PTRF
Gene ID (NCBI) 284119
RRIDAB_2935513
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6NZI2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Polymerase I and transcript release factor(PTRF) is an essential factor in the biogenesis of caveolae, which involve in numerous process, such as signal transduction and membrane and lipid trafficking. PTRF can terminate transcription of RNA Pol I, which involves pausing of transcription by TTF1, and dissociation of the transcription complex, release the complex from the template. The calculated molecular weight of PTRF is 43 kDa, but modified PTRF is about 50-55 kDa. (PMID: 11139612 )

实验方案

Product Specific Protocols
WB protocol for PTRF antibody 30086-1-APDownload protocol
IHC protocol for PTRF antibody 30086-1-APDownload protocol
IF protocol for PTRF antibody 30086-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols