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验证数据展示

  • WB analysis of multi-cells/tissue using 55123-1-AP (same clone as 55123-1-PBS)

    WB analysis of multi-cells/tissue using 55123-1-AP (same clone as 55123-1-PBS)

    WB result of 55123-1-AP. This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 55123-1-AP (same clone as 55123-1-PBS)

    WB analysis of K-562 using 55123-1-AP (same clone as 55123-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 55123-1-AP (PTPRJ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 55123-1-AP (same clone as 55123-1-PBS)

    IHC staining of human tonsillitis using 55123-1-AP (same clone as 55123-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55123-1-AP (PTPRJ Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 55123-1-AP (same clone as 55123-1-PBS)

    IHC staining of human tonsillitis using 55123-1-AP (same clone as 55123-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55123-1-AP (PTPRJ Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

  • WB analysis using 55123-1-AP (same clone as 55123-1-PBS)

    WB analysis using 55123-1-AP (same clone as 55123-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55123-1-AP (PTPRJ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 55123-1-AP (same clone as 55123-1-PBS)

    WB analysis of HeLa using 55123-1-AP (same clone as 55123-1-PBS)

    WB result of PTPRJ antibody (55123-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPRJ transfected HeLa cells. This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PTPRJ Polyclonal antibody, PBS Only

PTPRJ Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 55123-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

DEP-1, DEP1, DEP 1, Density-enhanced phosphatase 1, Density enhanced phosphatase 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

55123-1-PBS targets PTPRJ in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, J
别名 DEP-1, DEP1, DEP 1, Density-enhanced phosphatase 1, Density enhanced phosphatase 1
计算分子量 146 kDa
观测分子量 146-170 kDa
GenBank蛋白编号NM_002843
基因名称 PTPRJ
Gene ID (NCBI) 5795
RRIDAB_10859259
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12913
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PTPRJ, also named as CD148 and DEP1, belongs to the protein-tyrosine phosphatase family and Receptor class 3 subfamily. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPRJ may contribute to the mechanism of contact inhibition of cell growth. PTPRJ is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 (PLCG1) and Linker for Activation of T Cells (LAT). It was also found to dephosphorylate PDGF beta receptor, and may be involved in UV-induced signal transduction. The antibody is specific to PTPRJ.

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