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验证数据展示

  • WB analysis of Jurkat using 19715-1-AP

    WB analysis of Jurkat using 19715-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 19715-1-AP

    IHC staining of human tonsillitis using 19715-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 19715-1-AP

    IHC staining of human tonsillitis using 19715-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 19715-1-AP

    IHC staining of human tonsillitis using 19715-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Jurkat using 19715-1-AP

    IF Staining of Jurkat using 19715-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PTPRCAP / CD45AP antibody (19715-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

PTPRCAP / CD45AP Polyclonal antibody

PTPRCAP / CD45AP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19715-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

PTPRCAP, PTPRC associated protein, Protein tyrosine phosphatase receptor type C-associated protein, Lymphocyte phosphatase-associated phosphoprotein, LPAP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, human spleen tissue
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19715-1-AP targets PTPRCAP / CD45AP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, C-associated protein
别名 PTPRCAP, PTPRC associated protein, Protein tyrosine phosphatase receptor type C-associated protein, Lymphocyte phosphatase-associated phosphoprotein, LPAP
计算分子量 21 kDa
观测分子量 28-30 kDa
GenBank蛋白编号NM_005608
基因名称 PTPRCAP
Gene ID (NCBI) 5790
RRIDAB_10860107
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14761
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPRCAP, also named as LPAP and CD45-AP, interacts with CD45/PTPRC.

实验方案

Product Specific Protocols
WB protocol for PTPRCAP / CD45AP antibody 19715-1-APDownload protocol
IHC protocol for PTPRCAP / CD45AP antibody 19715-1-APDownload protocol
IF protocol for PTPRCAP / CD45AP antibody 19715-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols