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验证数据展示

  • WB analysis using 24143-1-AP

    WB analysis using 24143-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 24143-1-AP (PTPN6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 24143-1-AP

    IHC staining of human ovary cancer using 24143-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 24143-1-AP (PTPN6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTPN6 antibody included

PTPN6 Polyclonal antibody

PTPN6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24143-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

SHP1, Protein-tyrosine phosphatase 1C, HPTP1C, Hematopoietic cell protein-tyrosine phosphatase, HCPH

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, HL-60 cells, Jurkat cells, K-562 cells, Raji cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PTPN6 Ready-To-Use IHC Kit. PTPN6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24143-1-AP targets PTPN6 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag21415

Product name: Recombinant human PTPN6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 506-598 aa of BC002523

Sequence: QYKFIYVAIAQFIETTKKKLEVLQSQKGQESEYGNITYPPAMKNAHAKASRTSSKHKEDVYENLHTKNKREEKVKKQRSADKEKSKGSLKRK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, non-receptor type 6
别名 SHP1, Protein-tyrosine phosphatase 1C, HPTP1C, Hematopoietic cell protein-tyrosine phosphatase, HCPH
计算分子量 597 aa, 68 kDa
观测分子量 63 kDa
GenBank蛋白编号BC002523
基因名称 PTPN6/SHP1
Gene ID (NCBI) 5777
RRIDAB_3085725
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDP29350
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for PTPN6 antibody 24143-1-APDownload protocol
WB protocol for PTPN6 antibody 24143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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