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验证数据展示

  • WB analysis using 19510-1-AP

    WB analysis using 19510-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19510-1-AP (PTPN20B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 19510-1-AP

    IHC staining of mouse spleen using 19510-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19510-1-AP (PTPN20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse tongue using 19510-1-AP

    IHC staining of mouse tongue using 19510-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 19510-1-AP (PTPN20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 19510-1-AP

    IF Staining of A431 using 19510-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PTPN20 antibody (19510-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

PTPN20 Polyclonal antibody

PTPN20 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19510-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, ELISA

别名

PTPN20B, Tyrosine-protein phosphatase non-receptor type 20, PTPN20A, hPTPN20, EC:3.1.3.48

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse tongue tissue, pig tongue tissue, rat tongue tissue
Positive IHC detected inmouse tongue tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19510-1-AP targets PTPN20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, non-receptor type 20B
别名 PTPN20B, Tyrosine-protein phosphatase non-receptor type 20, PTPN20A, hPTPN20, EC:3.1.3.48
计算分子量 48 kDa
观测分子量52 kDa
GenBank蛋白编号NM_015605
基因名称 PTPN20B
Gene ID (NCBI) 26095
RRIDAB_3669310
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ4JDL3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN20B, also named as PTPN20A, belongs to the protein-tyrosine phosphatase family and Non-receptor class subfamily. PTPN20B is a tyrosine-protein phosphatase targeted to sites of actin polymerization in response of varied extracellular stimuli. PTPN20B has tyrosine phosphatase activity towards various tyrosyl phosphorylated substrates. PTPN20B catalyze the reaction: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. The antibody can recognize the isoform 1,2,7,8,10,11,13,15.

实验方案

Product Specific Protocols
WB protocol for PTPN20 antibody 19510-1-APDownload protocol
IHC protocol for PTPN20 antibody 19510-1-APDownload protocol
IF protocol for PTPN20 antibody 19510-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols