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验证数据展示

  • WB analysis using 85915-1-RR (same clone as 85915-1-PBS)

    WB analysis using 85915-1-RR (same clone as 85915-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85915-1-RR (PTPN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85915-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP02187-2

    Sandwich ELISA standard curve of MP02187-2

    Sandwich ELISA standard curve of MP02187-2, Human PTPN2 Recombinant Matched Antibody Pair - PBS only. 85915-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1709. 85915-1-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

  • Sample test of MP02187-2

    Sample test of MP02187-2

    The mean PTPN2 concentration was determined to be 102.95 ng/mL in HeLa cell extract based on a 1.30 mg/mL extract load and 169.33 ng/mL in HepG2 cell extract based on a 0.90 mg/mL extract load.

  • IF Staining of HeLa using 85915-1-RR (same clone as 85915-1-PBS)

    IF Staining of HeLa using 85915-1-RR (same clone as 85915-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTPN2 antibody (85915-1-RR, Clone: 250281G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85915-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP02187-2

    Cytometric bead array standard curve of MP02187-2

    Cytometric bead array standard curve of MP02187-2, PTPN2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85915-2-PBS. Detection antibody: 85915-1-PBS. Standard: Ag1709. Range: 0.938-120 ng/mL.

PTPN2 Recombinant antibody, PBS Only (Detector)

PTPN2 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85915-1-PBS

产品说明书

CloneNo. 250281G2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

EC:3.1.3.48, PTN2, PTPN 2, PTPT, TC PTP

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PTPN2 Ready-To-Use IHC Kit. PTPN2 primary antibody included.

产品信息

85915-1-PBS targets PTPN2 as part of a matched antibody pair:

MP02187-2: 85915-2-PBS capture and 85915-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 PTPN2 fusion protein Ag1709 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein tyrosine phosphatase, non-receptor type 2
别名 EC:3.1.3.48, PTN2, PTPN 2, PTPT, TC PTP
计算分子量 49 kDa
观测分子量45 kDa
GenBank蛋白编号BC016727
基因名称 PTPN2
Gene ID (NCBI) 5771
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP17706
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PTPN2(Tyrosine-protein phosphatase non-receptor type 2) is also named as PTPT and belongs to the protein-tyrosine phosphatase family. It may modulate beta-cell apoptosis and/or local release of inflammatory mediators in the course of a viral infection by acting, at least in part, at the pancreatic beta-cell level(PMID: 19825843). This protein has some isoforms produced by alternative splicing with the molecular mass of 40-50 kDa.