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验证数据展示

  • WB analysis using 67388-1-Ig

    WB analysis using 67388-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67388-1-Ig (PTPN2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67388-1-Ig

    WB analysis using 67388-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67388-1-Ig (PTPN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67388-1-Ig

    IHC staining of human breast cancer using 67388-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67388-1-Ig (PTPN2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTPN2 antibody included
  • IHC staining of human breast cancer using 67388-1-Ig

    IHC staining of human breast cancer using 67388-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67388-1-Ig (PTPN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTPN2 antibody included

PTPN2 Monoclonal antibody

PTPN2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67388-1-Ig

产品说明书

CloneNo. 2A1D1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

2A1D1, EC:3.1.3.48, PTN2, PTPN 2, PTPT

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, HeLa cells, 4T1 cells, LNCaP cells, HEK-293T cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, HepG2 cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PTPN2 Ready-To-Use IHC Kit. PTPN2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67388-1-Ig targets PTPN2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PTPN2 fusion protein Ag29749 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, non-receptor type 2
别名 2A1D1, EC:3.1.3.48, PTN2, PTPN 2, PTPT
计算分子量 49 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC016727
基因名称 PTPN2
Gene ID (NCBI) 5771
RRIDAB_2882632
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP17706
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN2(Tyrosine-protein phosphatase non-receptor type 2) is also named as PTPT and belongs to the protein-tyrosine phosphatase family. It may modulate beta-cell apoptosis and/or local release of inflammatory mediators in the course of a viral infection by acting, at least in part, at the pancreatic beta-cell level(PMID: 19825843). This protein has some isoforms produced by alternative splicing with the molecular mass of 40-50 kDa.

实验方案

Product Specific Protocols
WB protocol for PTPN2 antibody 67388-1-IgDownload protocol
IHC protocol for PTPN2 antibody 67388-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols