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验证数据展示

  • WB analysis using 67388-1-Ig

    WB analysis using 67388-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67388-1-Ig (PTPN2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67388-1-Ig

    WB analysis using 67388-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67388-1-Ig (PTPN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67388-1-Ig

    IHC staining of human breast cancer using 67388-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67388-1-Ig (PTPN2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTPN2 antibody included
  • IHC staining of human breast cancer using 67388-1-Ig

    IHC staining of human breast cancer using 67388-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67388-1-Ig (PTPN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTPN2 antibody included

PTPN2 Monoclonal antibody

PTPN2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67388-1-Ig

产品说明书

CloneNo. 2A1D1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

2A1D1, EC:3.1.3.48, PTN2, PTPN 2, PTPT

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells, HeLa cells, 4T1 cells, LNCaP cells, HEK-293T cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, HepG2 cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PTPN2 Ready-To-Use IHC Kit. PTPN2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67388-1-Ig targets PTPN2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag29749

Product name: Recombinant human PTPN2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-346 aa of BC016727

Sequence: MPTTIEREFEELDTQRRWQPLYLEIRNESHDYPHRVAKFPENRNRNRYRDVSPYDHSRVKLQNAENDYINASLVDIEEAQRSYILTQGPLPNTCCHFWLMVWQQKTKAVVMLNRIVEKESVKCAQYWPTDDQEMLFKETGFSVKLLSEDVKSYYTVHLLQLENINSGETRTISHFHYTTWPDFGVPESPASFLNFLFKVRESGSLNPDHGPAVIHCSAGIGRSGTFSLVDTCLVLMEKGDDINIKQVLLNMRKYRMGLIQTPDQLRFSYMAIIEGAKCIKGDSSIQKRWKELSKEDLSPAFDHSPNKIMTEKYNGNRIGLEEEKLTGDRCTGLSSKMQDTMEENSE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, non-receptor type 2
别名 2A1D1, EC:3.1.3.48, PTN2, PTPN 2, PTPT
计算分子量 49 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC016727
基因名称 PTPN2
Gene ID (NCBI) 5771
RRIDAB_2882632
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP17706
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN2(Tyrosine-protein phosphatase non-receptor type 2) is also named as PTPT and belongs to the protein-tyrosine phosphatase family. It may modulate beta-cell apoptosis and/or local release of inflammatory mediators in the course of a viral infection by acting, at least in part, at the pancreatic beta-cell level(PMID: 19825843). This protein has some isoforms produced by alternative splicing with the molecular mass of 40-50 kDa.

实验方案

Product Specific Protocols
WB protocol for PTPN2 antibody 67388-1-IgDownload protocol
IHC protocol for PTPN2 antibody 67388-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols