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PTPN11/SHP2 Polyclonal antibody

PTPN11/SHP2 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 24570-1-AP

Print datasheet

Synonyms

BPTP3, CFC, NS1, PTP 1D, PTP 2C, PTP2C, PTPN11, SH PTP2, SH PTP3, SHP 2, SHP2, SHPTP2

验证数据展示

  • IHC staining of human gliomas using 24570-1-AP

    IHC staining of human gliomas using 24570-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24570-1-AP (PTPN11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 24570-1-AP

    IHC staining of human gliomas using 24570-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24570-1-AP (PTPN11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HEK-293 using 24570-1-AP

    WB analysis of HEK-293 using 24570-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24570-1-AP (PTPN11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 24570-1-AP

    IHC staining of human skeletal muscle using 24570-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24570-1-AP (PTPN11 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 24570-1-AP

    IHC staining of human skeletal muscle using 24570-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24570-1-AP (PTPN11 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of MCF-7 using 24570-1-AP

    IF Staining of MCF-7 using 24570-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 24570-1-AP (PTPN11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, U-937 cells
Positive IHC detected inhuman skeletal muscle tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

24570-1-AP targets PTPN11/SHP2 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivitymouse
Immunogen PTPN11/SHP2 fusion protein Ag21472 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 11
Synonyms BPTP3, CFC, NS1, PTP 1D, PTP 2C, PTP2C, PTPN11, SH PTP2, SH PTP3, SHP 2, SHP2, SHPTP2
Calculated Molecular Weight 597 aa, 68 kDa
Observed Molecular Weight 68 kda
GenBank Accession NumberBC008692
Gene Symbol PTPN11
Gene ID (NCBI) 5781
RRIDAB_2879613
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ06124
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PTPN11/SHP2 antibody 24570-1-APDownload protocol
IHC protocol for PTPN11/SHP2 antibody 24570-1-APDownload protocol
IF protocol for PTPN11/SHP2 antibody 24570-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Oncol Lett

Curcumin inhibits proliferation of hepatocellular carcinoma cells by blocking PTPN1 and PTPN11 expression

Authors - Jingru Zhang
View Article