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验证数据展示

  • IF Staining of HeLa using 68582-1-Ig (same clone as 68582-1-PBS)

    IF Staining of HeLa using 68582-1-Ig (same clone as 68582-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTPIP51 antibody (68582-1-Ig, Clone: 1G11E2 ) at dilution of 1:476 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68582-1-PBS in a different storage buffer formulation.

  • WB analysis using 68582-1-Ig (same clone as 68582-1-PBS)

    WB analysis using 68582-1-Ig (same clone as 68582-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68582-1-Ig (PTPIP51 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68582-1-PBS in a different storage buffer formulation.

PTPIP51 Monoclonal antibody, PBS Only

PTPIP51 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68582-1-PBS

产品说明书

CloneNo. 1G11E2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

FAM82A2, 1G11E2, FAM82C, hRMD 3, hRMD-3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PTPIP51 Ready-To-Use IHC Kit. PTPIP51 primary antibody included.

产品信息

68582-1-PBS targets PTPIP51 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag15135

Product name: Recombinant human PTPIP51 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-141 aa of BC008970

Sequence: ESDNERDSDKESEDGEDEVSCETVKMGRKDSLDLEEEAASGASSALEAGGSSGLEDVLPLLQQADELHRGDEQGKREGFQLLLNNKLVYGSRQDFLWRLARAYSDMCELTEEVSEKKSYALDGKEEAEAALEKGDESADCH

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 family with sequence similarity 82, member A2
别名 FAM82A2, 1G11E2, FAM82C, hRMD 3, hRMD-3
计算分子量 470 aa, 52 kDa
观测分子量52-60 kDa
GenBank蛋白编号BC008970
基因名称 PTPIP51
Gene ID (NCBI) 55177
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ96TC7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein tyrosine phosphatase-interacting protein 51 (PTPIP51),also known as RMDN3, is a mitochondrial membrane-anchored protein that interacts with ER membrane-bound VAPB. The VAPB-PTPIP51 interaction impacts on intracellular Ca2+ handling (PMID: 22131369, 28345618). PTPIP51 participates in multiple cellular processes, and dysfunction of PTPIP51 is implicated in diseases such as cancer and neurodegenerative disorders (PMID: 28345618).The known initiation sites in the Ptpip51 gene would lead to molecular protein masses of 52, 38 and 30 kDa (PMID: 18854601,19844996).

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