验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, Jurkat cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human paracancerous of skin cancer, human cervical cancer tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:100-1:1200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20641-1-AP targets PTPIP51 in WB, IHC, IF, IP, COIP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, COIP |
经测试反应性 | human |
文献引用反应性 | human, mouse, rat, monkey |
免疫原 | PTPIP51 fusion protein Ag14708 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | family with sequence similarity 82, member A2 |
别名 | FAM82A2, Protein FAM82A2, hucep-10, hRMD-3, hRMD 3 |
计算分子量 | 470 aa, 52 kDa |
观测分子量 | 52-60 kDa |
GenBank蛋白编号 | BC008970 |
基因名称 | PTPIP51 |
Gene ID (NCBI) | 55177 |
RRID | AB_10695187 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q96TC7 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tyrosine phosphatase-interacting protein 51 (PTPIP51),also known as RMDN3, is a mitochondrial membrane-anchored protein that interacts with ER membrane-bound VAPB. The VAPB-PTPIP51 interaction impacts on intracellular Ca2+ handling (PMID: 22131369, 28345618). PTPIP51 participates in multiple cellular processes, and dysfunction of PTPIP51 is implicated in diseases such as cancer and neurodegenerative disorders (PMID: 28345618). The known initiation sites in the Ptpip51 gene would lead to molecular protein masses of 52, 38 and 30 kDa (PMID: 18854601,19844996).
实验方案
Product Specific Protocols | |
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WB protocol for PTPIP51 antibody 20641-1-AP | Download protocol |
IHC protocol for PTPIP51 antibody 20641-1-AP | Download protocol |
IP protocol for PTPIP51 antibody 20641-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Nat Cell Biol MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.
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Mol Cell The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation. | ||
J Cell Biol Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy. | ||
EMBO Rep Nup358 restricts ER-mitochondria connectivity by modulating mTORC2/Akt/GSK3β signalling
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J Cell Sci The role of Mfn2 in the structure and function of endoplasmic reticulum-mitochondrial tethering in vivo. |