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PTPIP51 Polyclonal antibody

PTPIP51 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 20641-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (3)

应用

WB, IHC, IP, ELISA and More (2)

FAM82A2, Protein FAM82A2, hucep-10, hRMD-3, hRMD 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman paracancerous of skin cancer, human cervical cancer tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20641-1-AP targets PTPIP51 in WB, IHC, IF, IP, COIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, COIP
经测试反应性 human
文献引用反应性human, mouse, rat, monkey
免疫原 PTPIP51 fusion protein Ag14708 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 82, member A2
别名 FAM82A2, Protein FAM82A2, hucep-10, hRMD-3, hRMD 3
计算分子量 470 aa, 52 kDa
观测分子量 52-60 kDa
GenBank蛋白编号BC008970
基因名称 PTPIP51
Gene ID (NCBI) 55177
RRIDAB_10695187
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96TC7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tyrosine phosphatase-interacting protein 51 (PTPIP51),also known as RMDN3, is a mitochondrial membrane-anchored protein that interacts with ER membrane-bound VAPB. The VAPB-PTPIP51 interaction impacts on intracellular Ca2+ handling (PMID: 22131369, 28345618). PTPIP51 participates in multiple cellular processes, and dysfunction of PTPIP51 is implicated in diseases such as cancer and neurodegenerative disorders (PMID: 28345618). The known initiation sites in the Ptpip51 gene would lead to molecular protein masses of 52, 38 and 30 kDa (PMID: 18854601,19844996).

实验方案

Product Specific Protocols
WB protocol for PTPIP51 antibody 20641-1-APDownload protocol
IHC protocol for PTPIP51 antibody 20641-1-APDownload protocol
IP protocol for PTPIP51 antibody 20641-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Metab

A High-Density Human Mitochondrial Proximity Interaction Network.

Authors - Hana Antonicka
monkeyWB,IF

Nat Cell Biol

MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.

Authors - Tim König
  • KD Validated
humanCOIP

Mol Cell

The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation.

Authors - Yan G Zhao
humanWB

J Cell Biol

Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy.

Authors - Nan Liu
humanWB,CoIP

EMBO Rep

Nup358 restricts ER-mitochondria connectivity by modulating mTORC2/Akt/GSK3β signalling

Authors - Misha Kalarikkal
  • KD Validated
humanWB

J Cell Sci

The role of Mfn2 in the structure and function of endoplasmic reticulum-mitochondrial tethering in vivo.

Authors - Song Han
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