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PRL3 Polyclonal antibody

PRL3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 15186-1-AP

Print datasheet

Synonyms

PRL 3, PRL R, PRL3, PTP4A3

验证数据展示

  • WB analysis using 15186-1-AP

    WB analysis using 15186-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15186-1-AP (PRL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 15186-1-AP

    IHC staining of human colon cancer using 15186-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 15186-1-AP

    IHC staining of human colon cancer using 15186-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 15186-1-AP

    IHC staining of human colon cancer using 15186-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human oesophagus cancer using 15186-1-AP

    IHC staining of human oesophagus cancer using 15186-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inhuman colon cancer tissue, human oesophagus cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15186-1-AP targets PRL3 in IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsIF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PRL3 fusion protein Ag7249 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein tyrosine phosphatase type IVA, member 3
Synonyms PRL 3, PRL R, PRL3, PTP4A3
Calculated Molecular Weight 20 kDa
Observed Molecular Weight25 kDa
GenBank Accession NumberBC003105
Gene Symbol PTP4A3
Gene ID (NCBI) 11156
RRIDAB_2878115
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75365
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phosphatase of regenerating liver-3 (PRL-3; also known as PTP4A3), encodes a 22-kDa protein that represents a novel class of a superfamily of protein tyrosine phosphatases (PTPs) that are ubiquitously expressed in various tumors. PRL3 was associated with primary tumor growth, tumor reoccurrence, and therapy resistance in many human cancers. This protein was also suggested as a marker to predict the relapse of gastric cancer and invasive breast cancer. (PMID: 22469786, PMID: 30157875)

实验方案

Product Specific Protocols
WB protocol for PRL3 antibody 15186-1-APDownload protocol
IHC protocol for PRL3 antibody 15186-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Reprod Sci

PRL-3 Is Involved in Estrogen- and IL-6-Induced Migration of Endometrial Stromal Cells From Ectopic Endometrium.

Authors - Shifan Ren
View Article
humanIF

Mol Cancer

Super-enhancer-driven TOX2 mediates oncogenesis in Natural Killer/T Cell Lymphoma

Authors - Jianbiao Zhou
View Article