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验证数据展示

  • WB analysis using 85075-1-RR

    WB analysis using 85075-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85075-1-RR (PTK7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85075-1-RR

    WB analysis using 85075-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85075-1-RR (PTK7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 85075-1-RR

    IHC staining of mouse brain using 85075-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85075-1-RR (PTK7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85075-1-RR

    Affinity and Kinetic Characterization of 85075-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85075-1-RR against Human PTK7 were performed. The affinity constant is 2.60 nM.

PTK7 Recombinant antibody

PTK7 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85075-1-RR

产品说明书

CloneNo. 242720A5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

242720A5, CCK 4, CCK4, CCK-4, Colon carcinoma kinase 4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, C2C12 cells, PC-12 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85075-1-RR targets PTK7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PTK7 fusion protein Ag12026 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PTK7 protein tyrosine kinase 7
别名 242720A5, CCK 4, CCK4, CCK-4, Colon carcinoma kinase 4
计算分子量 1070 aa, 118 kDa
观测分子量140 kDa
GenBank蛋白编号BC071557
基因名称 PTK7
Gene ID (NCBI) 5754
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13308
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tyrosine kinase 7 (PTK7), also known as colon carcinoma kinase 4 (CCK4), is an atypical receptor tyrosine kinase that consists of an extracellular domain with seven immunoglobulin-like domains, a transmembrane domain, and a catalytically defective tyrosine kinase domain (PMID: 29867084). It is implicated in planar cell polarity and the Wnt canonical and non-canonical pathways (PMID: 24618420). Overexpression of PTK7 has been reported in a variety of tumors, such as cervical cancer, colon cancer, lung adenocarcinoma, acute myelogenous leukemia, and gastric cancer (PMID: 30944666).

实验方案

Product Specific Protocols
WB protocol for PTK7 antibody 85075-1-RRDownload protocol
IHC protocol for PTK7 antibody 85075-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols