- Featured Product
- KD/KO Validated
BRK Polyclonal antibody
BRK Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, MCF-7 cells, rat spleen tissue, mouse spleen tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
18697-1-AP targets BRK in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IHC, IP, WB,ELISA |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | PTK6 protein tyrosine kinase 6 |
Synonyms | Breast tumor kinase, BRK, Protein tyrosine kinase 6, PTK6, PTK6 protein tyrosine kinase 6, Tyrosine protein kinase BRK |
Calculated Molecular Weight | 52 kDa |
Observed Molecular Weight | 52 kDa |
GenBank Accession Number | BC035843 |
Gene Symbol | PTK6 |
Gene ID (NCBI) | 5753 |
RRID | AB_2284691 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q13882 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for BRK antibody 18697-1-AP | Download protocol |
IHC protocol for BRK antibody 18697-1-AP | Download protocol |
IF protocol for BRK antibody 18697-1-AP | Download protocol |
IP protocol for BRK antibody 18697-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cancer Res RSK Promotes Prostate Cancer Progression in Bone through ING3, CKAP2 and PTK6-mediated Cell Survival.
| ||
Chin J Cancer The expression and prognostic value of protein tyrosine kinase 6 in early-stage cervical squamous cell cancer. | ||
Dose Response Genome-Wide Identification of Autophagy Prognostic Signature in Pancreatic Cancer. | ||
Adv Sci (Weinh) ETV4 Mediated Tumor-Associated Neutrophil Infiltration Facilitates Lymphangiogenesis and Lymphatic Metastasis of Bladder Cancer
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