The mean PYK2 concentration was determined to be 2.98 ng/mL in A431 cell extract based on a 1.5 mg/mL extract load, 0.71 ng/mL in HEK-293 cell extract based on a 1.5 mg/mL extract load, 3.03 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load and 1.66 ng/mL in THP-1 cell extract based on a 1.2 mg/mL extract load.

Sandwich ELISA standard curve of MP03196-1, Human PYK2 Recombinant Matched Antibody Pair - PBS only. 87746-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag11487. 87746-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

The mean PYK2 concentration was determined to be 24.2 ng/mL in THP-1 cell extract based on a 1.2 mg/mL extract load, 13.2 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load, 13.2 ng/mL in Daudi cell extract based on a 1.2 mg/mL extract load.

Cytometric bead array standard curve of MP03196-1, PYK2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87746-3-PBS. Detection antibody: 87746-2-PBS. Standard: Ag11487. Range: 0.781-100 ng/mL.