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验证数据展示

  • WB analysis using 67141-1-Ig (same clone as 67141-1-PBS)

    WB analysis using 67141-1-Ig (same clone as 67141-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67141-1-Ig (PYK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67141-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67141-1-Ig (same clone as 67141-1-PBS)

    IHC staining of mouse brain using 67141-1-Ig (same clone as 67141-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67141-1-Ig (PYK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67141-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PTK2B antibody included
  • IHC staining of mouse brain using 67141-1-Ig (same clone as 67141-1-PBS)

    IHC staining of mouse brain using 67141-1-Ig (same clone as 67141-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67141-1-Ig (PYK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67141-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PTK2B antibody included

PYK2 Monoclonal antibody, PBS Only

PYK2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67141-1-PBS

产品说明书

CloneNo. 2A10G5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, Indirect ELISA

别名

PTK2B, 2A10G5, CADTK, CAK beta, CAKB

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PTK2B Ready-To-Use IHC Kit. PTK2B primary antibody included.

产品信息

67141-1-PBS targets PYK2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag11487

Product name: Recombinant human PTK2B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 646-1009 aa of BC042599

Sequence: KPDLCPPVLYTLMTRCWDYDPSDRPRFTELVCSLSDVYQMEKDIAMEQERNARYRTPKILEPTAFQEPPPKPSRPKYRPPPQTNLLAPKLQFQVPEGLCASSPTLTSPMEYPSPVNSLHTPPLHRHNVFKRHSMREEDFIQPSSREEAQQLWEAEKVKMRQILDKQQKQMVEDYQWLRQEEKSLDPMVYMNDKSPLTPEKEVGYLEFTGPPQKPPRLGAQSIQPTANLDRTDDLVYLNVMELVRAVLELKNELCQLPPEGYVVVVKNVGLTLRKLIGSVDDLLPSLPSSSRTEIEGTQKLLNKDLAELINKMRLAQQNAVTSLSEECKRQMLTASHTLAVDAKNLLDAVDQAKVLANLAHPPAE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 PTK2B protein tyrosine kinase 2 beta
别名 PTK2B, 2A10G5, CADTK, CAK beta, CAKB
计算分子量 1009 aa, 116 kDa
观测分子量 112-115 kDa
GenBank蛋白编号BC042599
基因名称 PTK2B
Gene ID (NCBI) 2185
RRIDAB_2882440
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ14289
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Proline-rich tyrosine kinase 2 (Pyk2; also known as CAK, RAFTK and CADTK) is a cytoplasmic tyrosine kinase implicated to play a role in several intracellular signaling pathways. It is expressed in many cells and tissues and migrated as a 130 kDa band in Western Blotting analysis. The size of PYK2 is 115 kDa, and it expressed in many cells and tissues and migrated as a 130 kDa band, but several lower molecular weight bands (75 kDa, 80 kDa, and 97 kDa) were seen in Western Blotting analysis, possibly due to proteolytic degradation.

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