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验证数据展示

  • WB analysis using 87076-3-RR (same clone as 87076-3-PBS)

    WB analysis using 87076-3-RR (same clone as 87076-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87076-3-RR (PTH1R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87076-3-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 87076-3-RR (same clone as 87076-3-PBS)

    WB analysis of HEK-293 using 87076-3-RR (same clone as 87076-3-PBS)

    WB result of PTH1R antibody (87076-3-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTH1R transfected HEK-293 cells. This data was developed using the same antibody clone with 87076-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02999-1

    Sandwich ELISA sample test of MP02999-1

    The mean PTH1R concentration was determined to be 63.56 ng/mL in 786-O cell extract based on a 1.20 mg/mL extract load and 57.87 ng/mL in HK-2 cell extract based on a 1.20 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02999-1

    Sandwich ELISA standard curve of MP02999-1

    Sandwich ELISA standard curve of MP02999-1, Human PTH1R Recombinant Matched Antibody Pair - PBS only. 87076-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg6501. 87076-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

PTH1R Recombinant monoclonal antibody, PBS Only (Capture)

PTH1R Uni-rAb® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA
Cat No. 87076-3-PBS

产品说明书

CloneNo. 252173F3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Sandwich ELISA, Indirect ELISA

别名

Parathyroid hormone/parathyroid hormone-related peptide receptor, PFE, PTHR, PTHR1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87076-3-PBS targets PTH1R as part of a matched antibody pair:

MP02999-1: 87076-3-PBS capture and 87076-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Eg6501

Product name: Recombinant Human PTH1R protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 27-188 aa of NM_000316.2

Sequence: DADDVMTKEEQIFLLHRAQAQCEKRLKEVLQRPASIMESDKGWTSASTSGKPRKDKASGKLYPESEEDKEAPTGSRYRGRPCLPEWDHILCWPLGAPGEVVAVPCPDYIYDFNHKGHAYRRCDRNGSWELVPGHNRTWANYSECVKFLTNETREREVFDRLG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 parathyroid hormone 1 receptor
别名 Parathyroid hormone/parathyroid hormone-related peptide receptor, PFE, PTHR, PTHR1
计算分子量66 kDa
观测分子量66 kDa and 90 kDa
GenBank蛋白编号NM_000316.2
基因名称 PTH1R
Gene ID (NCBI) 5745
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ03431
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PTH1R is a receptor for parathyroid hormone and parathyroid hormone-related peptide (PTHrP). PTH1R is a glycoprotein with seven transmembrane domains and it has an extracellular N-terminus region and an intracellular C-terminus region. The activity of PTH1R is mediated by G proteins which activate adenylyl cyclase. It has been reported that PTHrP and PTH1R are associated with the differentiation of bone and cartilage in the developing stages. The calculated molecular weight of PTH1R is 66 kDa, and PTH1R can form a 120 kDa dimer in the non-reducing condition. With glycosylation modification, the molecular weight of PTH1R will migrate to 90 kDa (PMID: 16783882).

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