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PTH1R Polyclonal antibody

PTH1R Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29115-1-AP

Print datasheet

Synonyms

parathyroid hormone 1 receptor, PFE, PTH/PTHr receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTH1R, PTHR, PTHR1

验证数据展示

  • WB analysis using 29115-1-AP

    WB analysis using 29115-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29115-1-AP (PTH1R antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 29115-1-AP

    IHC staining of human pancreas cancer using 29115-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29115-1-AP (PTH1R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, mouse kidney tissue, HepG2 cells, mouse liver tissue, rat kidney tissue, rat liver tissue
Positive IHC detected inhuman pancreas cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29115-1-AP targets PTH1R in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen PTH1R fusion protein Ag30762 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name parathyroid hormone 1 receptor
Synonyms parathyroid hormone 1 receptor, PFE, PTH/PTHr receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTH1R, PTHR, PTHR1
Calculated Molecular Weight 593 aa, 66 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberBC110388
Gene Symbol PTH1R
Gene ID (NCBI) 5745
RRIDAB_2918237
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ03431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTH1R is a receptor for parathyroid hormone and parathyroid hormone-related peptide (PTHrP). PTH1R is a glycoprotein with seven transmembrane domains and it has an extracellular N-terminus region and an intracellular C-terminus region. The activity of PTH1R is mediated by G proteins which activate adenylyl cyclase. It has been reported that PTHrP and PTH1R are associated with the differentiation of bone and cartilage in the developing stages. PTH1R has several isoforms with 66/59 kDa and PTH1R can form a 120 kDa dimer in the non-reducing condition.

实验方案

Product Specific Protocols
WB protocol for PTH1R antibody 29115-1-APDownload protocol
IHC protocol for PTH1R antibody 29115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols