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验证数据展示

  • WB analysis using 33863-1-AP (same clone as 33863-1-PBS)

    WB analysis using 33863-1-AP (same clone as 33863-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33863-1-AP (CD315/PTGFRN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33863-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 33863-1-AP (same clone as 33863-1-PBS)

    IP experiment of HeLa using 33863-1-AP (same clone as 33863-1-PBS)

    IP result of anti-CD315/PTGFRN (IP:33863-1-AP, 4ug; Detection:33863-1-AP 1:300) with HeLa cells lysate 1480 ug. This data was developed using the same antibody clone with 33863-1-PBS in a different storage buffer formulation.

CD315/PTGFRN Polyclonal antibody, PBS Only

CD315/PTGFRN Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 33863-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, Indirect ELISA

别名

PTGFRN, CD315, EWIF, FPRP, KIAA1436

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

33863-1-PBS targets CD315/PTGFRN in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg5010

Product name: Recombinant Human PTGFRN/CD315 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 26-832 aa of BC152454

Sequence: VPTATLVRVVGTELVIPCNVSDYDGPSEQNFDWSFSSLGSSFVELASTWEVGFPAQLYQERLQRGEILLRRTANDAVELHIKNVQPSDQGHYKCSTPSTDATVQGNYEDTVQVKVLADSLHVGPSARPPPSLSLREGEPFELRCTAASASPLHTHLALLWEVHRGPARRSVLALTHEGRFHPGLGYEQRYHSGDVRLDTVGSDAYRLSVSRALSADQGSYRCIVSEWIAEQGNWQEIQEKAVEVATVVIQPSVLRAAVPKNVSVAEGKELDLTCNITTDRADDVRPEVTWSFSRMPDSTLPGSRVLARLDRDSLVHSSPHVALSHVDARSYHLLVRDVSKENSGYYYCHVSLWAPGHNRSWHKVAEAVSSPAGVGVTWLEPDYQVYLNASKVPGFADDPTELACRVVDTKSGEANVRFTVSWYYRMNRRSDNVVTSELLAVMDGDWTLKYGERSKQRAQDGDFIFSKEHTDTFNFRIQRTTEEDRGNYYCVVSAWTKQRNNSWVKSKDVFSKPVNIFWALEDSVLVVKARQPKPFFAAGNTFEMTCKVSSKNIKSPRYSVLIMAEKPVGDLSSPNETKYIISLDQDSVVKLENWTDASRVDGVVLEKVQEDEFRYRMYQTQVSDAGLYRCMVTAWSPVRGSLWREAATSLSNPIEIDFQTSGPIFNASVHSDTPSVIRGDLIKLFCIITVEGAALDPDDMAFDVSWFAVHSFGLDKAPVLLSSLDRKGIVTTSRRDWKSDLSLERVSVLEFLLQVHGSEDQDFGNYYCSVTPWVKSPTGSWQKEAEIHSKPVFITVKMDVLNAFKYP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 prostaglandin F2 receptor negative regulator
别名 PTGFRN, CD315, EWIF, FPRP, KIAA1436
计算分子量 879 aa, 99 kDa
观测分子量 100-130 kDa
GenBank蛋白编号BC152454
基因名称 PTGFRN
Gene ID (NCBI) 5738
RRIDAB_3743099
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9P2B2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PTGFRN (Prostaglandin F2 Receptor Negative Regulator), also known as CD315, is a trans-membrane protein that modulates prostaglandin F2α signaling and links the tetraspanin web to the cytoskeleton, it is now recognized as a driver of metastasis; high expression correlates with aggressive proliferation, migration, and spheroid growth in epidermoid carcinoma, medulloblastoma, and mesothelioma, while silencing PTGFRN impairs these traits and disrupts autophagy (PMID: 38924562). The calculated value of PTGFRN is 98 kDa. It has multiple glycosylation sites and its molecular weight after glycosylation is approximately 100 - 130 kDa.

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