验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21595-1-AP targets PTGFR in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag16173 Product name: Recombinant human PTGFR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 305-359 aa of BC112965 Sequence: ILLRKAVLKNLYKLASQCCGVHVISLHIWELSSIKNSLKVAAISESPVAEKSAST 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | prostaglandin F receptor (FP) |
| 别名 | FP, PGF receptor, PGF2 alpha receptor, prostaglandin F receptor (FP), Prostanoid FP receptor |
| 计算分子量 | 359 aa, 40 kDa |
| 观测分子量 | 30-40 kDa |
| GenBank蛋白编号 | BC112965 |
| 基因名称 | PTGFR |
| Gene ID (NCBI) | 5737 |
| RRID | AB_3742046 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P43088 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Prostanoid FP receptor(PTGFR) is a member of the G-protein coupled receptor family and is involved in several physiologic processes. PTGFR is a receptor for prostaglandin F2-alpha (PGF2-alpha), which is known to be a potent luteolytic agent, and may also be involved in modulating intraocular pressure and smooth muscle contraction in the uterus(PMID: 9235889).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PTGFR antibody 21595-1-AP | Download protocol |
| WB protocol for PTGFR antibody 21595-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
