PTGES3 Monoclonal antibody

PTGES3 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat and More (1)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3C11D11

Cat No : 67736-1-Ig

Print datasheet

Synonyms

cPGES, Hsp90 co chaperone, P23, Prostaglandin E synthase 3, PTGES3, TEBP, Telomerase binding protein p23



经过测试的应用

Positive WB detected inA549 cells, PC-3 cells, HeLa cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PTGES3 Ready-To-Use IHC Kit. PTGES3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

67736-1-Ig targets PTGES3 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, mouse, pig, rat
Cited Reactivityhuman
Immunogen PTGES3 fusion protein Ag7870 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name prostaglandin E synthase 3 (cytosolic)
Synonyms cPGES, Hsp90 co chaperone, P23, Prostaglandin E synthase 3, PTGES3, TEBP, Telomerase binding protein p23
Calculated Molecular Weight 19 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC003005
Gene Symbol PTGES3
Gene ID (NCBI) 10728
RRIDAB_2918505
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15185
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

P23, encoded by the gene PTGES3, plays a key role in glucocorticoid signaling and was increased at the mRNA level in the DLPFC in individuals with schizophrenia (PMID: 24345775). In the GR heterocomplex in vitro, p23 is an obligatory co-factor19 and is the limiting component of the complex44, functioning to stabilize the interaction of the complex with GR in the cytoplasm. Paradoxically, p23 also acts in the nucleus to inhibit GR-mediated gene transcription and disassemble GR transcriptional machinery in the nucleus (PMID: 12077419). In vivo, p23 is critical for appropriate glucocorticoid responsiveness (PMID: 17000766).

实验方案

Product Specific Protocols
WB protocol for PTGES3 antibody 67736-1-IgDownload protocol
IHC protocol for PTGES3 antibody 67736-1-IgDownload protocol
IF protocol for PTGES3 antibody 67736-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int Immunopharmacol

High expression of PTGES3 is an independent predictive poor prognostic biomarker and correlates with immune infiltrates in lung adenocarcinoma.

Authors - Pengxiang Gao
humanWB

Cell Death Discov

METTL9-SLC7A11 axis promotes hepatocellular carcinoma progression through ferroptosis inhibition

Authors - Fangfang Bi
humanWB

J Transl Med

The integration of multidisciplinary approaches revealed PTGES3 as a novel drug target for breast cancer treatment

Authors - Qinan Yin